Page 55 - 南京医科大学自然版

第44卷第5期 南京医科大学学报（自然科学版）
2024年5月 Journal of Nanjing Medical University（Natural Sciences） ·643 ·

·临床研究·

结肠癌中ALOX12表达与临床病理特征及预后的关系分析

付志强 ，高启行 ，郭文文 ，何震宇 1*
2
1
1
南京医科大学第二附属医院普外科，病理科，江苏 南京 210011
1 2

［摘 要］ 目的：探讨花生四烯酸 12⁃脂氧合酶（arachidonate 12⁃lipoxygenase，ALOX12）在结肠癌患者癌组织中的表达及意
义。方法：回顾性收集257例结肠癌患者肿瘤及瘤旁组织，免疫组化法检测ALOX12的表达，比较肿瘤及瘤旁组织内表达差异
并分析其表达与患者临床病理特征的相关性。随访并探讨 ALOX12 表达与患者 5 年总生存率的关系，并采用 Kaplan⁃Meier
法绘制生存曲线。运用单因素及多因素 Cox 回归综合分析影响结肠癌患者 5 年总生存率的因素。结果：与瘤旁组织相比，
ALOX12在肿瘤中阳性表达率更高（63.8% vs. 33.1%），差异具有统计学意义（P < 0.001）。ALOX12 表达与患者年龄、性别、肿瘤
部位均无明显相关性（P > 0.05），而与TNM分期、淋巴结转移有显著相关性（P < 0.05）。ALOX12高表达患者的5年总生存率明
显低于低表达患者（P < 0.001）。此外，单因素和多因素Cox回归分析结果显示年龄、TNM分期、淋巴结转移、ALOX12表达是影
响患者5年总生存率的独立影响因素（P < 0.05）。结论：ALOX12高表达与结肠癌TNM分期、淋巴结转移相关，可作为预测患
者病情及预后的潜在指标。
［关键词］ 结肠癌；花生四烯酸12⁃脂氧合酶；铁死亡；临床病理特征；预后
［中图分类号］ R735.3 ［文献标志码］ A ［文章编号］ 1007⁃4368（2024）05⁃643⁃06
doi：10.7655/NYDXBNSN240134

Analysis on the relationship of ALOX12 expression with clinicopathological features and
prognosis in colon cancer

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1
1
FU Zhiqiang ，GAO Qihang ，GUO Wenwen ，HE Zhenyu 1*
1 Department of General Surgery，Department of Pathology，the Second Affiliated Hospital of Nanjing Medical
2
University，Nanjing 210011，China
［Abstract］ Objective：To explore the expression and significance of arachidonate 12⁃lipoxygenase（ALOX12）in cancer tissues of
colon cancer patients. Methods：Tumor and peritumoral tissues of 257 colon cancer patients were retrospectively collected，and the
expression of ALOX12 was detected by immunohistochemistry（IHC）to compare the differences in expression within the tumor and
peritumoral tissues，and the relationship between ALOX12 expression and clinicopathological features of colon cancer was analyzed.
The 5⁃year overall survival（OS）of the patients was analyzed using Kaplan⁃Meier curves，and the factors affecting the 5⁃year OS of the
patients were investigated by univariate and multivariate Cox regression analyses. Results：ALOX12 was more positively expressed in
tumors compared to peritumoral tissues（63.8% vs. 33.1%），and the difference was statistically significant（P < 0.001）. The expression
of ALOX12 was not significantly correlated with the patient’s age，gender，and tumor site（P > 0.05），but was strongly correlated with
TNM staging and lymph node metastasis（P < 0.05）. Five⁃year OS of patients with high expression of ALOX12 was markedly lower than
those patients with low expression of ALOX12（P < 0.001）. Single variable and multivariate Cox analyses indicated that age，TNM
staging，lymph node metastasis，and ALOX12 expression were independent factors influencing the 5 ⁃ year OS rate（P < 0.05）.
Conclusion：High expression of ALOX12 is associated with TNM staging and lymph node metastasis of colon cancer，which can serve
as a biomarker to predict the patient’s condition and prognosis.
［Key words］ colon cancer；arachidonate 12⁃lipoxygenase；ferroptosis；clinicopathological feature；prognosis
［J Nanjing Med Univ，2024，44（05）：643⁃648］

［基金项目］ 江苏省卫生健康委医学科研项目（K2023019）
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通信作者（Corresponding author），E⁃mail：hezhenyu1968@163.com