Page 93 - 南京医科大学自然版

第44卷第5期 南京医科大学学报（自然科学版）
2024年5月 Journal of Nanjing Medical University（Natural Sciences） ·681 ·

·临床研究·

线粒体DNA含量在结直肠癌中的预后价值

杜杨斌 ，郭文文 ，何震宇 1*
2
1
南京医科大学第二附属医院普外科，病理科，江苏 南京 210011
1 2

［摘 要］ 目的：探究组织线粒体DNA（mitochondrial DNA，mtDNA）含量和结直肠癌预后相关性。方法：选取117例结直肠癌
患者并收集临床病理资料。运用RT⁃qPCR检测患者癌组织与癌旁组织mtDNA含量，探究mtDNA含量与各项预后指标的相关
性。绘制受试者工作特征（receiver operating characteristic，ROC）曲线，根据截断值区分患者并绘制无病生存期（disease free sur⁃
vival，DFS）曲线。单因素和多因素Cox回归分析探究术后DFS相关的危险因素。结果：与癌旁组织相比，癌组织mtDNA 含量
差异无统计学意义（P=0.432）；低mtDNA含量与肿瘤位于结肠、低分化、TNM分期差、淋巴结转移相关（P < 0.05）。ROC曲线提
示 mtDNA 含量为 500.699 可作为截断值。单因素和多因素分析显示，mtDNA 含量低于 500.699（HR=4.285，95%CI：1.938~
9.475）、肿瘤低分化（HR=2.886，95%CI：1.428~5.835）是与DFS相关的独立危险因素。结论：结直肠癌患者中，组织mtDNA含量
与临床病理特征有关，低mtDNA含量是患者预后相关的独立危险因素。
［关键词］ 线粒体DNA；结直肠癌；预后；肿瘤复发；无病生存期
［中图分类号］ R735 ［文献标志码］ A ［文章编号］ 1007⁃4368（2024）05⁃681⁃07
doi：10.7655/NYDXBNSN231165

Prognostic value of mitochondrial DNA content in colorectal cancer
1
2
DU Yangbin ，GUO Wenwen ，HE Zhenyu 1*
1 Department of General Surgery，Department of Pathology，the Second Affiliated Hospital of Nanjing Medical
2
University，Nanjing 210011，China

［Abstract］ Objective：To investigate the prognostic correlation between mitochondrial DNA（mtDNA）content and colorectal cancer.
Methods：Clinical and pathological data were collected from 117 patients with colorectal cancer. RT⁃qPCR was used to determine the
mtDNA content of cancer tissues and paracancerous tissues of patients，and the correlation between mtDNA content and various
prognostic indicators was investigated. Receiver operating characteristic（ROC）curves were plotted，and the cut ⁃ off value was
determined to distinguish patients，and disease free survival（DFS）curves were plotted accordingly. Univariate and multivariate Cox
regression analyses were conducted to explore the risk factors related to postoperative DFS. Results：There was no significant
difference in mtDNA content between cancer tissues and paracancerous tissues（P=0.432）；low mtDNA content was associated with
tumor location in the colon，poor differentiation，advanced TNM stage，and lymph node metastasis（P < 0.05）. The ROC curve indicated
a cut⁃off value of 500.699 for mtDNA content. Univariate and multivariate Cox analyses showed that mtDNA content lower than 500.699
（HR=4.285，95%CI：1.938-9.475）and poorly differentiated tumors（HR=2.886，95%CI：1.428-5.835）were independent risk factors
associated with DFS. Conclusion：In patients with colorectal cancer，tissue mtDNA content is associated with clinical and pathological
characteristics，and low mtDNA content is an independent risk factor for patient prognosis.
［Key words］ mitochondrial DNA；colorectal cancer；prognosis；tumor recurrence；disease⁃free survival
［J Nanjing Med Univ，2024，44（05）：681⁃687］

［1］
结直肠癌（colorectal cancer，CRC）作为全球第 3 大最常见的恶性肿瘤，是癌症死亡的第2大原因 。
目前，伴随着中国经济逐渐发展，CRC 发病率迅速
［2］
［基金项目］ 南京科技发展计划项目（2018SC512012） 上升 。手术切除以及术后辅助治疗是Ⅱ期或Ⅲ期
∗ CRC 患者的标准治疗方法，但仍有超过 30%的Ⅱ期
通信作者（Corresponding author），E⁃mail：hezhenyu1968@163.com