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A
CPO⁃PCL
Control 0.10% 0.25% 0.50% 1.00% Normoxia and osteogenic Normoxia and normal
differentiation culture
culture
Osteogenic differentiation culture 4 3 *** *** 4 3 ***
RUNX2 Relative fluorescence intensity of RUNX2 2 1 Relative fluorescence intensity of RUNX2 2 1 ** * ***

Normal culture 0 Control 0.10% 0.25% 0.50% 1.00% 0 Control 0.10% 0.25% 0.50% 1.00%

CPO⁃PCL CPO⁃PCL
B CPO⁃PCL
Control 0.10% 0.25% 0.50% 1.00% Normoxia and osteogenic Normoxia and normal
culture
differentiation culture
Osteogenic differentiation culture 2.5 ** ** 5 4 ** ***
2.0
Osteocalcin Relative fluorescence intensity of Osteocalcin 1.5 Relative fluorescence intensity of Osteocalcin 3 2 1 ** **
1.0
Normal culture 0 Control 0.10% 0.25% 0.50% 1.00% 0 Control 0.10% 0.25% 0.50% 1.00%
0.5

CPO⁃PCL CPO⁃PCL
C CPO⁃PCL
Control 0.10% 0.25% 0.50% 1.00%
Normoxia and osteogenic Normoxia and normal
differentiation culture
culture
Osteogenic differentiation culture 2.0 4 3 *** ***
1.5
Osteopontin Relative fluorescence intensity of Osteopontin 1.0 Relative fluorescence intensity of Osteopontin 2 1 *
Normal culture 0 Control 0.10% 0.25% 0.50% 1.00% 0 Control 0.10% 0.25% 0.50% 1.00%
0.5

CPO⁃PCL CPO⁃PCL
Effects of different concentrations of CPO⁃PCL on microparticles RUNX2（A），Osteocalcin（B）and Osteopontin（C）expression after normoxic osteo⁃
**
*
genic differentiation or normal culture for 14 days（Scale bars=100 μm；magnification：×200）. Compared with the control group，P < 0.05，P < 0.01 and
***
P < 0.001（n=3）.
图10 免疫荧光检测常氧14 d不同浓度CPO⁃PCL 微粒对ADMSC成骨分化培养和正常培养中RUNX2、Osteocalcin 和 Os⁃
teopontin的表达情况
Figure 10 Expression levels of RUNX2，Osteocalcin and Osteopontin in ADMSC with osteogenic differentiation culture or
normal culture after treatment of different concentrations of CPO⁃PCL microparticles under normoxia for 14
days detected by immunofluorescence

治疗骨缺损的新方法奠定了基础。 ［3］ XING M，WANG X，WANG E，et al. Bone tissue engineer⁃

ing strategy based on the synergistic effects of silicon and
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