Page 11 - 南京医科大学自然版

第44卷第12期 程 丽，倪 曼，王 洁，等. 环磷酰胺对卵母细胞发育潜能影响的研究［J］.
2024年12月 南京医科大学学报（自然科学版），2024，44（12）：1621-1628，1648 ·1625 ·

A Mock Ctrl 4⁃HC
Cumulus cell expansion

PB1

100 μm 100 μm 100 μm

Blastocyst

100 μm 100 μm 100 μm
* *
B * C D
200 * 1.0 * 1.0 *
0.8
0.8
（μm） 150 in vitro 0.6 0.6
Diameter 100 Maturation rate of GV oocyte 0.4 Blastocyst rate 0.4

50
0.2
0.2
0 0 0
Mock Ctrl 4⁃HC Mock Ctrl 4⁃HC Mock Ctrl 4⁃HC
A：Compared with the Ctrl group，the expansion of cumulus cells in COC treated with 1.0 μmol/L 4⁃HC was poor，the extrusion rate of the first polar
body（PB1）was decreased，and the formation of blastocyst was decreased（scale bar=100 μm）. B：Cumulus cell diffusion diameter in each group. C：The
*
PB1 extrusion rate of each group. D：Blastocyst rate of each group. P < 0.05（n=60）.
图2 1.0 μmol/L 4⁃HC作用下COC体外成熟培养结果
Figure 2 In vitro maturationresults of COC treated with 1.0 μmol/L 4⁃HC

A Mock Ctrl 4⁃HC B 250 ** **
Relative fluorescence intensity of GSH 150
200

GSH 100
50
0
Mock Ctrl 4⁃HC
150 μm 150 μm 150 μm C **
100 *
Relative fluorescence intensity of DHE 60
80

DHE 40

20
0
150 μm 150 μm 150 μm Mock Ctrl 4⁃HC
A：ROS staining fluorescence of MⅡ stage oocytes treated with 1.0 μmol/L 4⁃HC（scale bar=150 μm）. B：Semi⁃quantitative GSH fluorescence
**
*
levels of oocytes in each group. C：Semi⁃quantitative DHE fluorescence levels of oocytes in each group. P < 0.05，P < 0.01（n=60）.
图3 1.0 μmol/L 4⁃HC作用下MⅡ期卵母细胞氧化应激水平
Figure 3 Oxidative stress levels in MⅡoocytes treated with 1.0 μmol/L 4⁃HC