Page 55 - 南京医科大学自然版

第45卷第10期 任 钰，李超普，张 许，等. 肥胖相关基因Stambpl1的生物信息学筛选及其对脂代谢影响
2025年10月 的初步探究［J］. 南京医科大学学报（自然科学版），2025，45（10）：1417-1426，1466 ·1423 ·

A 2.0
1.5
1.0
log2FC 0.5
0
-0.5
-1.0
Stambpl1 Slc15a3 Myo1f Top2a Mki67 Atp6v0d2

B
Stambpl1 Slc15a3 Myo1f
1.0 1.0 1.0
0.8 0.8 0.8
Sensitivity 0.6 AUC：1.000 Sensitivity 0.6 AUC：0.625 Sensitivity 0.6 AUC：0.625
0.4
0.4
0.4
0.2 0.2 0.2
0 0 0
0 0.2 0.4 0.6 0.8 1.0 0 0.2 0.4 0.6 0.8 1.0 0 0.2 0.4 0.6 0.8 1.0
1-Specificity 1-Specificity 1-Specificity
Top2a Mki67 Atp6v0d2
1.0 1.0 1.0
0.8
0.8
0.8
Sensitivity 0.6 AUC：0.750 Sensitivity 0.6 AUC：0.750 Sensitivity 0.6 AUC：0.500
0.4
0.4
0.4
0.2 0.2 0.2
0 0 0
0 0.2 0.4 0.6 0.8 1.0 0 0.2 0.4 0.6 0.8 1.0 0 0.2 0.4 0.6 0.8 1.0
1-Specificity 1-Specificity 1-Specificity
C 15 *** iWAT 5 eWAT **
mRNA expression 2.0 5 ** ND mRNA expression 4 3 * ND
10
HFD
HFD

Relative 1.5 0 Relative 2 1
1.0
0.5
Stambpl1 Plin1 0 Stambpl1 Plin1
A：The logarithmic scale fold changes of hub genes in GSE138632. B：Draw the ROC curve of hub genes using GSE138632. C：RT⁃PCR was used
to detect the expression of Stambpl1 and Plin1 in iWAT（n=5）and eWAT（n=6）of C57BL/6J male mice fed with HFD diet for 12 weeks. Double
***
*
**
tailed t⁃test analysis was performed（ P < 0.05，P < 0.01， P < 0.001）.
图3 在数据集和小鼠脂肪组织中的验证hub gene
Figure 3 Validation of hub genes in datasets and mouse adipose tissue

A 5 *** B 20 ***
Stambpl1 protein relative 2
NC OE 4 mRNA relative 15

Stambpl1 50 kDa expression level 3 expression level 10
Calnexin 90 kDa 1 Stambpl1 5
0
NC OE 0 NC OE

A：The overexpression efficiency of Stambpl1 detected by Western blot using Calnexin as an internal reference. B：The overexpression efficiency of
***
Stambpl1 detected by RT⁃PCR（n=3， P < 0.001）.
图4 构建Stambpl1过表达细胞系
Figure 4 Construction of Stambpl1 overexpression cell line