Page 53 - 南京医科大学自然版

第45卷第10期 任 钰，李超普，张 许，等. 肥胖相关基因Stambpl1的生物信息学筛选及其对脂代谢影响
2025年10月 的初步探究［J］. 南京医科大学学报（自然科学版），2025，45（10）：1417-1426，1466 ·1421 ·

A GSE37218 GSE30247 B
10
6
GSE37218 GSE30247
5 8
4
P P 6
-lg 3 -lg 4 72 57 378
2
2
1
0 0
-4 -2 0 2 4 -4 -2 0 2 4
log2FC log2FC
C Heterotypic cell⁃cell adhesion 3
Inflammatory response 6
Mitotic spindle organization 4
Negative regulation of macrophage migration 2
Positive regulation of leukocyte cell⁃cell adhesion 3
Positive regulation of cell migration 6
Actin⁃based cell projection 4
Microvillus 3
Extracellular membrane⁃bounded organelle 3
Extracellular vesicle 3
Tertiary granule membrane 3
Chemoattractant activity 3 Biological process
Cellular component
Cytokine receptor activity 3 Molecular function
Chemokine binding 2
0 1 2 3 4
-lg P
D MGI mammalian phenotype level Human phenotype level
Increased susceptibility to bacterial infection Leukocytosis
Increased interleukin⁃10 secretion
Abnormality of eosinophils
Abnormal osteoclast differentiation
Follicular hyperkeratosis
Abnormal cellular extravasation
Nephrotic syndrome
Decreased CD4⁃positive，alpha⁃beta T cell number
Type 1 muscle fiber predominance
Abnormal neutrophil physiology
Slender build
Abnormal leukocyte adhesion Ankle contracture
No abnormal phenotype detected
Enriched pathways Decreased susceptibility to experimental Enriched pathways Slow progression
Recurrent gram⁃negative bacterial infections
Abnormal response to cardiac infarction
Decreased T cell proliferation
Severe combined immunodeficiency
Decreased germinal center B cell number
Joint hemorrhage
Abnormal CD4⁃positive，alpha⁃beta T cell physiology
Hemiplegia
Autoimmune encephalomyelitis
Decreased CD8⁃positive，alpha⁃beta T cell number
Combined immunodeficiency
Decreased spleen germinal center size Tubular atrophy
Periodontitis
Decreased interleukin⁃6 secretion
Increased variability in muscle fiber diameter
Increased NK T cell number
Abnormality of the vocal cords
Increased acute inflammation
Mildly elevated creatine phosphokinase
Decreased skeletal muscle weight
T lymphocytopenia
Decreased acute inflammation Eosinophilia
1.5 2.0 2.5 3.0 3.5 2.0 3.0 4.0
Enrichment score（-lg P） Enrichment score（-lg P）
A：Gene volcano map after differential analysis of GSE37218 and GSE30247 using GEO2R. B：Venn diagram of up⁃regulated genes in GSE37218
and GSE30247（P < 0.05，log2FC > 1.5）. C：GO enrichment map of common differentially expressed genes in GSE37218 and GSE30247. The numbers
adjacent to the bars denote the count of genes enriched in the corresponding GO terms. D：Enrichment analysis of mouse and human phenotypes of
common differentially expressed genes in GSE37218 and GSE30247.
图1 差异基因的筛选及功能表型的富集分析
Figure 1 Screening of differentially expressed genes and enrichment analysis of functional phenotypes
需要利用可靠且具有临床相关性的实验模型 ［17］ 。
3 讨 论
C57BL/6J小鼠常被用于研究食物诱发的肥胖症、糖
肥胖已成为严重威胁人类健康的问题，而脂肪 尿病和动脉粥样硬化等代谢性疾病 ［18］ 。因此，本研
组织在机体能量代谢中发挥着至关重要的作用 ［16］ 。 究在前期选取了 HFD 诱导的 C57BL/6J 小鼠 eWAT
生物信息学的应用为深入探究肥胖症的发病机制、 测序数据来研究肥胖症发生过程中的潜在分子机
寻找潜在治疗靶点提供了有力工具。 制。GEO数据库是一个国际公共资源库，目前已接
为了解肥胖相关代谢性疾病发生的分子机制， 受来自各种技术的研究数据，包括 DNA 微阵列、蛋