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200 ABC⁃2
180 ABC⁃17
ABC⁃18
160 ABC⁃56
Fluorescence intensity 120 ssDNA pool
140
ABC⁃82
100
80
60
40
20
0
SKBR3 MCF⁃7 MDA⁃MB⁃231 MCF⁃10A SGC⁃7901 A549 SW480 A2780 HepG2 PANC⁃1
图5 DNA适配子与各种腺癌细胞的结合情况
Figure 5 The binding of DNA aptamers to various adenocarcinoma cells
DAPI ABC⁃18 Merge DAPI ABC⁃56 Merge
MCF⁃7 MCF⁃7
MDA⁃MB⁃231 MDA⁃MB⁃231
图6 激光共聚焦显微镜观察DNA适配子与乳腺癌细胞株的结合（×200）
Figure 6 DNA aptamers bind to breast cancer cell lines by confocal laser microscope（×200）
×1 000 ×1 000 ×1 000
A B C
×1 000 ×400 ×400
D E F
Analysiss of immunofluorescence staining. A⁃C：Breast cancer tissues with positive binding，showing Luminal A（A），Luminal B（B）and HER2（C）
subtype，respectively. D：Breast cancer tissues with negative binding（triple negative subtype）. E，F：Breast fibroadenoma tissues（E）and normal breast
tissues（F）with low or negative binding.
图7 免疫荧光显微镜观察适配子ABC⁃56与乳腺来源的各种组织的结合
Figure 7 Binding of aptamer ABC⁃56 to tissues from breast by immunofluorescence microscope
