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A

Tissue Magnetic
dissociation beads Cell sorting Elution 1 10
Nucleotide
co⁃
incubation
RNA sequencing

B C
Group Metalion binding
3M⁃HD DNA binding
（×10 ） 3M⁃WT
4
Extracellular region
8 Count
Regulation of transcription by RNA polymerasell 10
Multicellular organism development 20
Proteolysis 30
Protein⁃containing complex -lg（P value）
（8.9%） 4 Monoalomicion transmembrane transport 3.0
Protein heterodimerization activity
2.5
Neuron differentiation
2.0
Dim2 Protein homooligomerization 1.5
Establishment of localization in cell
Heparin binding
-4
High⁃density lipoprotein partide
Stereocllium
10 20 30
5
-2 -1 0 1 2（×10 ） Count
Dim1（81.9%）
D E
Group Extracellular region
Down（122） Extracellular space
4
Non（17 595） Monoatomicion transport
Up（197） Calciumion binding Count
value） 3 Basolateral plasma membrane 5 10
Inflammatory response
15
（P 2 Response to lipopclysaccharide -lg（P value）
Postsynaptic membrane
-lg Epidermis development
1 Synaptic cleft 5
4
Receptor ligand activity
3
Spermidine transport
0 2
Maintenance of synapse structure
-10 -5 0 5 10 Atriated muscle contraction
log2 （Fold change） Spermidine transmembrane transporter activity
5 10 15
Count
F
Group
HD
WT
Expression
2
1
0
-1
-2
C2
Adam18 Ascl2 Trim71 Six4 Tdrd9 Tdrd1 Tssk4 Satb2 Dppa5a Fezf1 Lmx1a Tll1 Ntf5 Htr3a Artn Cbln1 Cbln3 Reg3b Ctsw Drd2 Cxcl13 Fst S100a14 Kcnj4 Napsa Sfrp2 Cga Tac1 Msr1

A：Schematic diagram of astrocyte isolation from mice brain. B：PCA analysis of 3⁃month⁃old mice in the HD group and WT group. C：The volcano
map represent the differential gene expressions. D：GO functional enrichment analysis of up⁃regulated DEGs. E：GO functional enrichment analysis of
down⁃regulated DEGs. F：The heatmap of synapse⁃related DEGs.
图2 3月龄HD小鼠星形胶质细胞转录组学差异分析
Figure 2 Differential transcriptome analysis of astrocytes in 3⁃month⁃old HD mice