Page 57 - 南京医科大学自然版

第45卷第4期 陈贝宁，杨清湖，袁增强，等. 亨廷顿舞蹈症中星形胶质细胞转录异质性分析［J］.
2025年4月 南京医科大学学报（自然科学版），2025，45（4）：487-497，587 ·493 ·

A B
Group
（×10 ） 12M⁃HD Down（237）
5
12M⁃WT 4
Non（15 338）
1 3 Up（2 295）
（11.9%） 0 value） （P 2

Dim2 -lg 1

-1 0
-10 -5 0 5 10
log2 （Fold change）
5
-2 0 2 （×10 ）
Dim1（79.9%）
C D
Signal transduction Inflammatory response
Cell differentiation Immune response
Cell adhesion Intracellular signal transduction
Inflammatory response Chemotaxis
Brain development Cell surface receptor signaling pathway
Positive regulation of apoptotic process Phagocytosis
Positive regulation of neruobtast proliferation Receptor⁃mediated endocytosis
Dendrite arborization Phagocytosis，engulfment
Glial cell differentiation Microglial cell activation
Blood vessel remodeling Astrocyte activation
Extracellular space Extracellutar space
Synapse Cytoskeleton
Cell surface External side of plasma membrane Ontology
Collagen⁃containing extracellular matrix Ontology Endosome
Axon BP Collagen⁃containing extracellular matrix BP
Presynapse CC Extracellular matrix CC
GABA⁃ergic synapse MF Membrane raft MF
Presynaptic membrane Vesicle
Perineuronal net Inner dynein arm
Perisynaptic extracellular matrix Phagocytic cup
Identical protein binding Protein binding
Oxidoreductase activity ATP binding
Calcium ion binding Calcium ion binding
Protein homodimerization activity Transmembrane signaling receptor activity
Signaling receptor binding Signaling receptor activity
Heparin binding Integrin binding
Carbohydrate binding Heparin binding
Neuropeptide receptor activity Ehemokine activity
Growth factor activity Endopeptidase activity
Molecular function activator activity Dynein intermediate chain binding
0 10 20 30 40 0 200 400
Count Count
E 483 GO terms clustered by‘binary_cut’
Positive activation inflammatory cell pathway innate
response signaling chemotaxis immune
Movement motility cilium
Beta necroaia expreaaion proliferation interterongamma
production positive regulation interleukin
Odontogenesis tissue pain Similarity
differentiation myoblast perception lung glomerular 0.8
development angiogenesis 0.6
0.4
Transmembrane secretion cilium exocytosis across
0.2
ion transport calcium phagocytosis potassium
0
Adhesion cellcell molecules leukocyte cell
Phosphorylation
Organization actin cytoskeleton assembly
Apoptotic process endothelial cell negative regutation
Response stimulus calcium ion cellutar
Proliferation cell population regutation astrocyts
Transmission glutamatergic synaptic signaling
A：PCA analysis of 12⁃month⁃old mice from the HD group and WT group. B：The volcano map represente the differential gene expressions. C：GO
functional enrichment analysis of up⁃regulated DEGs. D：GO functional enrichment analysis of down⁃regulated DEGs. E：The clustering of GO terms of
up⁃regulated DEGs. BP：biological process；CC：cellular component；MF：molecular function.
图3 12月龄HD小鼠星形胶质细胞转录组学差异分析
Figure 3 Differential transcriptome analysis of astrocytes in 12⁃month⁃old HD mice