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A CRC_GSE166555 THBS2
4.0
Plasma
Cell type（major⁃lineage） 3.5
B
Fibroblasts CD4Tconv 3.0
CD8T
Epithelia DC
CD4TconV 2.5
Endothelial
Myofibroblasts
CD8T Epithelial
Fibroblasts 2.0
Malignant Tprolif Malignant
Mast
Mast 1.5
Mono/Macro
Myofibroblasts
Mono/Macro 1.0
DC Plasma
Endothelial Tprolif
0.5
B
0
B C FAP THBS2
（log2 TPM） 15 Cancer associated fibroblast_EPIC
r=0.931
P<0.001
expression level 10 COAD 100 μm DAPI 100 μm Merge

THBS2 5 0

0 0.25 0.50 0.75 1.00
Infiltration level
100 μm 100 μm

D NF CAF 6 NF
Relative expression of protein
Vimentin 70 kDa * * CAF

α⁃SMA 45 kDa 4

FAP 100 kDa 2 *

GAPDH 35 kDa 0
Vimentin α⁃SMA FAP
F P=0.04
4 800
Relative THBS2 expression 2 400
E *
NF CAF 3 expression 600
THBS2 130 kDa

GAPDH 35 kDa 1 Relative THBS2 200

0 0
NF CAF NF CAF
A：UMAP clustering plot（left）and UMAP clustering plot showing cellular localization of THBS2 expression（right）in single⁃cell sequencing dataset
GSE166555（n=12）. B：Correlation analysis between THBS2 and CAFs in COAD using the TIMER2.0 database（n=458）. C：Expression and spatial
localization of THBS2 and FAP in clinical CRC tissue samples detected by multiplex immunohistochemistry（n=5）. D：Western blot analysis of CAF
markers（Vimentin，α⁃SMA，FAP）in NF and CAF（n=3）. E：Western blot analysis of THBS2 expression in NF and CAF（n=3）. F：ELISA quantification
*
of THBS2 levels in conditioned medium from NF and CAF（n=5）. P < 0.05.
图2 THBS2主要由CAF分泌产生
Figure 2 THBS2 was mainly secreted by CAF