Page 28 - 《南京医科大学学报（自然科学版）》2026年第3期

第46卷第3期
·336 · 南 京 医 科 大 学 学 报 2026年3月

PSM分析结果显示胃癌组织中CLEC5A蛋白表 的表达与 N 分期、TNM 期（P < 0.05）和 CEA 水平
达（27/36，75.0%）显著高于正常胃黏膜组织（13/36， （P < 0.01）显著相关（表1）。
36.1%），差异有统计学意义（P < 0.05），且胃癌组的 2.2 CLEC5A蛋白表达量与胃癌患者预后和总体生
高表达率较高（图1C）。CLEC5A蛋白表达高低与胃 存的相关性
癌患者的临床特征的关系如表1所示。统计结果表 Cox 回归模型单因素分析显示，肿瘤组织中
明，CLEC5A 的表达与 T 分期、N 分期和术前外周血 CLEC5A 蛋白的表达（P < 0.001）、间质淋巴细胞中
癌胚抗原（carcinoembryonic antigen，CEA）水平显著 CLEC5A蛋白的表达（P < 0.001）、TNM分期（P < 0.001）、
相关（P 均 < 0.05）。间质淋巴细胞中 CLEC5A 蛋白 T 分 期（P < 0.001）、N 分 期（P < 0.001）、M 分 期

A C
5 P<0.001
4
Normal tissues
3
2
1
0
Normal Tumor a1 a2 a3
B Overall survival
1.0 Low CLEC5A group（n=192）
High CLEC5A group（n=192）
logrank P=0.64
0.8 HR（high）=1.10
Percent survival 0.6 P（HR）=0.64 GC tissues b1 b2 50 μm b3
0.4
0.2
50 μm
0
c1 c2 c3
0 20 40 60 80 100 120
Time（months）
D E
Survival functions Survival functions
1.0 CLEC5A in cancer 1.0 TNM
0 1
1 2
0.8 0.Censored 0.8 3 1.censored
1.Censored
Overall survival 0.6 Overall survival 0.6 2.censored
3.censored
0.4
0.4
0.2 0.2
0 0
0 20 40 60 0 20 40 60
Time（months） Time（months）
A：Gastric cancer（GC）patient datasets were downloaded from TCGA included 375 tumor tissues，and the mRNA expression of CLEC5A in
GC tissues was significantly higher than that paracancerous normal samples（n=407）. B：Kaplan⁃Meier analysis showed CLEC5A expression was not
correlated with prognosis（n=407）. C：Representative IHC results of CLEC5A expression in normal gastric mucosal tissues and GC tissues. a1：Normal
gastric mucosal tissues showed different degrees of CLEC5A staining. a2，a3：The staining scores of normal gastric mucosal tissues and lymphocytes
were determined by the automatic reading system. b1：GC tissues showed different degrees of CLEC5A staining. b2，b3：The staining scores of GC
tissues and TILs were determined by the automatic reading system. a1，b1：Original image for scanning recognition（scale bar=50 μm）. D：Survival analysis
of GC patients according to CLEC5A expression by Kaplan⁃Meier analysis. GC patients with higher CLEC5A expression in tumor cells（green line，1）
have a better OS，compared with those with low or no CLEC5A expression in tumor cells（blue line，0）（n=145）. E：The OS was significantly better in
patients with early TNM stage compared with patients with advanced TNM stage（n=145）.
图1 CLEC5A在胃癌中的表达及其生存曲线评估预后价值
Figure 1 Expression of CLEC5A in GC and its prognostic value evaluated by survival curve