Page 31 - 南京医科大学自然版

第46卷第7期 付太冉，孙传峰，咸 峰. 布比卡因脂通过线粒体钙信号轴影响老年麻醉患者认知功能的机制［J］.
2026年7月 南京医科大学学报（自然科学版），2026，46（7）：973-982 ·977 ·

Anesthesia Baseline
Baseline 1 d 3 d 5 d 7 d 2.0 Anesthesia 1 d
Target protein expression 1.0 #▲ #▲
p⁃Ser637⁃Drp1 82 kDa Anesthesia 3 d * *
Anesthesia 5 d
p⁃Ser616⁃Drp1 82 kDa 1.5 Anesthesia 7 d #▲
Drp1 80 kDa *

β⁃actin 42 kDa 0.5 * *
mito⁃Drp1 80 kDa
0
p⁃Ser637⁃Drp1 p⁃Ser616⁃Drp1 Drp1 mito⁃Drp1
mito⁃COX Ⅳ 20 kDa
*
#
▲
Compared with the baseline，P < 0.05；compared with 1 day after BPV anesthesia，P < 0.05；compared with 3 days after BPV anesthesia，P < 0.05
（n=3）.
图2 BPV麻醉后小鼠海马中的Drp1、p⁃Ser637⁃Drp1、p⁃Ser616⁃Drp1和线粒体中mito⁃Drp1蛋白表达
Figure 2 Drp1，p⁃Ser637⁃Drp1，p⁃Ser616⁃Drp1 protein expression in the hippocampus of BPV anesthetized mice，and mito⁃
Drp1 protein expression in mitochondria

BPV Con BPV 12 h
Con 1 h 3 h 6 h 12 h 24 h 2.5 BPV 1 h BPV 24 h *#▲
Target protein expression
p⁃Ser637⁃Drp1 82 kDa 2.0 BPV 3 h *
BPV 6 h
p⁃Ser616⁃Drp1 82 kDa 1.5 *
Drp1 80 kDa 1.0 *

β⁃actin 42 kDa 0.5 * *
mito⁃Drp1 80 kDa 0
p⁃Ser637⁃Drp1 p⁃Ser616⁃Drp1 Drp1 mito⁃Drp1
mito⁃COX Ⅳ 20 kDa
*
▲
#
Compared with the control group，P < 0.05；compared with the BPV 1 h group，P < 0.05；compared with BPV 3 h，P < 0.05（n=3）.
图3 原代神经元中的Drp1、p⁃Ser637⁃Drp1、p⁃Ser616⁃Drp1和线粒体中mito⁃Drp1蛋白表达
Figure 3 Drp1，p⁃Ser637⁃Drp1，p⁃Ser616⁃Drp1 protein expression in primary neurons and mito⁃Drp1 protein expression in mi-
tochondria
0.05）。BPV+ASM981 组原代神经元中 p⁃AMPK、 2.5 钙调神经磷酸酶介导 BPV 对原代神经元细胞
mito⁃Drp1 蛋白水平和钙调神经磷酸酶显著低于 线粒体功能的影响
BPV组（P < 0.05），p⁃Ser637⁃Drp1蛋白水平显著高于 通过荧光显微镜下的MitoTracker Red标记观察
BPV组（P < 0.05，图4）。 神经元的线粒体图像。与 Con 组相比，BPV 组原代

A B 3 Con 6
BPV
Con BPV ASM981 BPV+ASM981 ASM981 * *
BPV+ASM981
p⁃AMPK 62 kDa 2 * * （nmol PO4/mg protein） 4
AMPK 62 kDa Target protein expression # Calcineurin phosphatase activity 2
p637⁃Drp1 82 kDa 1 # # #
β⁃actin 42 kDa * * 0 *
0
mito⁃Drp1 80 kDa
p⁃AMPK AMPK p637⁃Drp1 mito⁃Drp1 Con BPV ASM981
mito⁃COX Ⅳ 20 kDa BPV+ASM981

A：Western blot analysis of the protein expression levels of p⁃AMPK，AMPK，p⁃Ser637⁃Drp1 in primary neurons，and mito⁃Drp1 in mitochondria.
*
B：Measurement of calcineurin activity in neurons following 12 h of BPV exposure. Compared with the Con group，P < 0.05；compared with the BPV
#
group，P < 0.05（n=3）.
图4 钙调神经磷酸酶介导的Drp1激活对原代神经元BPV刺激的反应
Figure 4 Response of Drp1 activation mediated by calcineurin to BPV stimulation in primary neurons