Page 39 - 南京医科大学自然版

第46卷第9期 魏甲园，徐聖业，朱妍妍，等. ReN细胞来源纳米囊泡中的let⁃7i⁃5p在帕金森病模型中通过抗凋亡作用
2026年9月 发挥保护效应［J］. 南京医科大学学报（自然科学版），2026，46（9）：1303-1314 ·1309 ·

A B C
250 8 **
***
（s） 200 （s） 6
Latency to fall Total descent time 4
150
RVG⁃nanovesicles Sacrificed 100 2

Day： 1 2 3 4 5 7 9 15 50 0 0
Control MPTP Control MPTP
MPTP
D TH RVG DAPI Merge

200 μm

50 μm
E TH Iba1 DAPI Merge F
1.5 **
Normalized fluorescence intencity of TH
Control 1.0 ***

100 μm 0.5

PBS 0 Control MPTP+RV
MPTP+RVG⁃RV
MPTP+PBS
100 μm
G
1.5 * *
Normalized fluorescence intencity of Iba1
MPTP RV 1.0

100 μm 0.5
RVG⁃RV 0 Control MPTP+RV
MPTP+PBS

100 μm MPTP+RVG⁃RV
A：Schematic illustration of the experimental design showing acute PD modeling via intraperitoneal injection of MPTP and subsequent tail vein
administration of nanovesicles. B：Rotarod test showing significantly reduced latency to fall in MPTP⁃treated mice compared with controls（n=6）. C：Pole
test showing significantly prolonged total descent time in the MPTP group（n=6）. D：Immunofluorescence co⁃localization of RVG⁃ReN⁃NV with TH⁃positive
cells. TH immunoreactivity（green）marks tyrosine hydroxylase⁃positive cells，RVG⁃ReN⁃NV is visualized by red fluorescence，with nuclei counter⁃
stained by DAPI（blue）（n=3）. E：Immunofluorescence staining of the substantia nigra in control，PBS，ReN⁃NV，or RVG⁃ReN⁃NV treated mice
following MPTP administration，showing TH（green）and Iba1（red）expression，with nuclei counterstained by DAPI（blue）. Scale bar：200 μm（n=3）.
F：Quantification of TH fluorescence intensity in the substantia nigra. G：Quantification of Iba1 fluorescence intensity in the substantia nigra. P < 0.05；
*
** ***
P < 0.01 and P < 0.001.
图2 ReN⁃NV和RVG⁃ReN⁃NV能够减轻MPTP造成的黑质区域的损伤
Figure 2 ReN⁃NV and RVG⁃ReN⁃NV can alleviate the damage in the substantia nigra region caused by MPTP