Page 36 - 南京医科大学学报自然科学版

南京医科大学学报（自然科学版） 第42卷第1期
· 30 · Journal of Nanjing Medical University（Natural Sciences） 2022年1月

·基础研究·

大鼠骨质疏松模型的建立及其骨髓间充质干细胞内差异lncRNA
初步分析

刘易昕，何 强，张大鹏，严 坤，姚年伟，钱卫庆 *

南京中医药大学附属南京中医院骨伤科，江苏 南京 210000

［摘 要］ 目的：分析骨质疏松模型大鼠骨髓间充质干细胞（bone marrow mesenchymal stem cell，BMSC）内差异长链非编码
RNA（long noncoding RNA，lncRNA）及相关功能。方法：SD大鼠分为骨质疏松模型组和对照组，每组6只，用切除卵巢的方法制
备骨质疏松模型，通过双能 X 线骨密度检测仪测定两组大鼠手术前及 3 个月后的骨密度。取 SD 大鼠股骨内骨髓体外培
养 BMSC，利用基因芯片技术检测骨质疏松模型组（3 只）和对照组（3 只）大鼠 BMSC 内 lncRNA 的表达差异，通过 GO 功能分
析及 KEGG 通路富集，分析差异 lncRNA 的生物学功能及相关信号通路。结果：骨质疏松模型组 SD 大鼠骨密度［（157.33±
6.28）mg/cm］，较对照组［（183.33±4.50）mg/cm］显著降低。两组大鼠 BMSC 内检测出基因片段共计 32 759 个，差异有统计学
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意义的有8 454个（P < 0.05），其中上调3 593个，下调4 861个。检测到的lncRNA 基因片段4 992个，差异有统计学意义的共
116 个（P < 0.05），其中上调 35 个，下调 81 个。差异 lncRNA 主要参与细胞生长发育生物学过程，并调控蛋白的转运通路。
结论：骨质疏松模型大鼠的BMSC内lncRNA 表达和正常大鼠存在差异，差异表达的lncRNA 参与对BMSC生长发育过程及蛋
白转运通路的调控。
［关键词］ 骨质疏松；骨髓间充质干细胞；lncRNA；基因芯片
［中图分类号］ R681 ［文献标志码］ A ［文章编号］ 1007⁃4368（2022）01⁃030⁃06
doi：10.7655/NYDXBNS20220105

Establishment of rat osteoporosis model and preliminary analysis of the differences of
lncRNA in BMSC cells of model rats

LIU Yixin，HE Qiang，ZHANG Dapeng，YAN Kun，YAO Nianwei，QIAN Weiqing *
Department of Orthopedic Surgery，Nanjing TCM Hospital Affiliated to Nanjing University of Traditional Chinese
Medicine，Nanjing 210000，China

［Abstract］ Objective：To analyze the differences of lncRNA in cultured bone marrow mesenchymal stem cells（BMSCs） in
osteoporotic model rats and the functions of the lncRNA. Methods：Twelve SD rats were equally divided into the osteoporosis model
group and the control group. The osteoporosis model was made by ovariectomy. Bone mineral density（BMD）in ovariectomized rats and
sham control were determined by dual⁃energy X⁃ray bone mineral density detector. BMSCs from femur bone marrow were cultured in
vitro. The different expression of lncRNA in BMSCs of osteoporosis model（n=3）and control group（n=3）was detected by gene chip
technology, and the different biological functions of lncRNA and related signaling pathway were analyzed by GO function analysis and
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the KEGG enrichment. Results：BMD in osteoporosis model rats［（157.33 ± 6.28）mg/cm］，was lower than the control group rats
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［（183.33±4.50）mg/cm］significantly（P < 0.05）. A total of 32 759 gene fragments were detected in BMSCs in the osteoporosis model
group and the control group, and 8 454 lncRNA gene fragments had significant difference（P < 0.05），among which 3 593 were up⁃
regulated and 4 861 were down⁃regulated in osteoporosis model group. Totally 4 992 lncRNA gene fragments were detected，and there
were significant differences in 116 lncRNA gene fragments in BMSCs of osteoporosis model rats，with 35 increased and 81 decreased.
Differential lncRNAs are mainly involved in the biological process of cell growth and development，and regulate protein transport
pathways. Conclusion：There were differences in the expression of lncRNA in BMSCs between osteoporosis model rats and normal rats,

［基金项目］ 南京市医学科技发展项目（YKK17155）
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通信作者（Corresponding author），E⁃mail：fsyy00274@njucm.edu.cn