Page 29 - 南京医科大学学报自然科学版

第43卷第5期 南京医科大学学报（自然科学版）
2023年5月 Journal of Nanjing Medical University（Natural Sciences） ·611 ·

·肿瘤专题研究·

LncRNA RP11⁃490M8.1 在乳腺癌中的表达及其对乳腺癌细胞

增殖的影响

杨 莹 ，王子璇 ，陈 狄 ，陈  ，方亚群 ，金玉翠 ，马长艳 ，陈 翔 2*
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南京医科大学基础医学院医学遗传学系，江苏 南京 211166；江苏大学附属宜兴市人民医院甲乳外科，江苏 宜兴
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214221
［摘 要］ 目的：确定lncRNA RP11⁃490M8.1在乳腺癌中的表达情况及其在乳腺癌细胞增殖中的作用。方法：基于癌症基因
图谱（The Cancer Genome Atlas，TCGA）数据库和Kaplan⁃Meier plotter数据库分析RP11⁃490M8.1在乳腺癌中的表达情况及与患
者预后的关系；qRT⁃PCR 检测RP11⁃490M8.1在乳腺癌和癌旁组织中的表达；CCK8和克隆形成实验检测过表达和敲低RP11⁃
490M8.1对乳腺癌细胞增殖的影响。结果：与癌旁组织相比，RP11⁃490M8.1在乳腺癌组织中表达明显下调，且RP11⁃490M8.1
低表达和较差的总生存期（overall survival，OS）密切相关（P=0.034）；RP11⁃490M8.1表达水平与乳腺癌患者的临床分期呈负相
关，而与雌激素受体、孕激素受体、人表皮生长因子受体⁃2的表达以及年龄均无相关性；过表达RP11⁃490M8.1显著抑制乳腺癌
细胞的增殖，敲低RP11⁃490M8.1表达则促进其增殖。结论：RP11⁃490M8.1在乳腺癌中可能发挥抑癌基因的作用，是潜在的乳
腺癌预后分子和治疗靶点。
［关键词］ RP11⁃490M8.1；乳腺癌；增殖；预后
［中图分类号］ R737.9 ［文献标志码］ A ［文章编号］ 1007⁃4368（2023）05⁃611⁃06
doi：10.7655/NYDXBNS20230504

Expression of lncRNA RP11⁃490M8.1 in breast cancer and its roles in the proliferation of
breast cancer cells
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YANG Ying ，WANG Zixuan ，CHEN Di ，CHEN Yan ，FANG Yaqun ，JIN Yucui ，MA Changyan ，CHEN Xiang 2*
Department of Medical Genetics，School of Basic Medical Sciences，Nanjing Medical University，Nanjing 211166；
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Department of Thyroid and Breast Surgery，Yixing People’s Hospital Affiliated to Jiangsu University，Yixing 214221，
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China
［Abstract］ Objective：To investigate the expression of lncRNA RP11⁃490M8.1 in patients with breast cancer and its roles in the
proliferation of breast cancer cells. Methods：The Cancer Genome Atlas（TCGA）and the Kaplan ⁃Meier plotter database were used to
define the expression level and prognostic value of RP11⁃490M8.1 in breast cancer，respectively. qRT⁃PCR was performed to detect the
expression level of RP11⁃490M8.1 in paired breast cancer tissues and adjacent normal tissues. CCK8 and clone formation assays were
carried out to determine the effects of the overexpression or knockdown of RP11⁃490M8.1 on the proliferation of breast cancer cells.
Results：Compared with adjacent normal tissues，RP11⁃490M8.1 was significantly downregulated in breast cancer tissues. Moreover，
lower expression of RP11 ⁃ 490M8.1 was associated with the advanced clinical stage and worse overall survival（OS）（P=0.034）.
However，there was no correlation between RP11⁃490M8.1 level and the expression of estrogen receptor，progesterone receptor，human
epidermal growth factor receptor⁃2 and age. Overexpression of RP11⁃490M8.1 markedly suppressed the proliferation of breast cancer
cells，while knockdown of RP11⁃490M8.1 promoted its proliferation. Conclusion：RP11⁃490M8.1 acts as a tumor suppressor in breast
cancer，which may serve as a potential prognostic molecule and therapeutic target for breast cancer.
［Key words］ RP11⁃490M8.1；breast cancer；proliferation；prognosis
［J Nanjing Med Univ，2023，43（05）：611⁃616］
［基金项目］ 国家自然科学基金（81872389）；新锐肿瘤支持治疗课题研究项目（cphcf⁃2022⁃121）；无锡市卫健委妇幼健康科研
项目（FYKY 201907）；江苏省高等学校大学生创新创业训练计划（202110312002Z）
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通信作者（Corresponding author），E⁃mail：cyma@njmu.edu.cn；staff984@yxph.com