Page 37 - 南京医科大学自然版

第44卷第4期 南京医科大学学报（自然科学版）
2024年4月 Journal of Nanjing Medical University（Natural Sciences） ·475 ·

·基础研究·

牛磺鹅去氧胆酸对兔实验性肠炎损伤的保护作用初探

钱鑫娜，朱文卿，邱 憬 *

南京医科大学附属口腔医院种植科，江苏省口腔疾病研究重点实验室，江苏省口腔转化医学工程研究中心，江苏 南京 210029

［摘 要］ 目的：分析牛磺鹅去氧胆酸（taurochenodeoxycholic acid，TCDCA）对肠道炎症的疗效，为肠道急慢性炎症的治疗提供
理论依据。方法：体内外实验分组均为对照组、脂多糖（lipopolysaccharide，LPS）组、LPS+TCDCA组。体外实验中首先应用MTT
法筛选TCDCA的适宜工作浓度，随后LPS组给予巨噬细胞LPS刺激，LPS+TCDCA组先后给予LPS与TCDCA刺激，RT⁃qPCR和
Western blot检测炎症相关mRNA和蛋白的表达。体内实验中，LPS组通过兔耳缘静脉注射LPS溶液，LPS+TCDCA组同上处理后
通过饮水喂食TCDCA溶液。通过HE染色、过碘酸⁃雪夫染色和阿尔新蓝⁃核固红染色评估小肠的组织病理学改变。结果：体
外实验结果显示，与LPS组相比，LPS+TCDCA组巨噬细胞中肿瘤坏死因子⁃α、白介素⁃1β、白介素⁃6、干扰素⁃γ表达量显著降低，
白介素⁃10表达量升高。体内实验中，HE染色结果显示小肠组织炎症缓解，过碘酸⁃雪夫染色和阿尔新蓝⁃核固红染色结果显
示LPS+TCDCA 组杯状细胞数量和酸性、中性黏蛋白分泌量均较LPS组增多。结论：TCDCA可通过降低炎症因子的表达缓解
兔肠道组织炎症，减轻LPS造成的肠道炎症损伤。
［关键词］ 牛磺鹅去氧胆酸；肠道损伤；巨噬细胞；脂多糖
［中图分类号］ R574 ［文献标志码］ A ［文章编号］ 1007⁃4368（2024）04⁃475⁃08
doi：10.7655/NYDXBNSN230741

The protective effect of taurochenodeoxycholic acid on experimental enteritis injury in
rabbits：a preliminary study

QIAN Xinna，ZHU Wenqing，QIU Jing *
Department of Oral Implantology，the Affiliated Stomatological Hospital of Nanjing Medical University，Jiangsu
Province Key Laboratory of Oral Diseases，Jiangsu Privince Engineering Research Center of Stomatological
Trarslational Medicine，Nanjing 210029，China

［Abstract］ Objective：To analyze the efficacy of taurochenodeoxycholic acid（TCDCA）on intestinal inflammation and to provide a
theoretical basis for the treatment of acute and chronic intestinal inflammation. Methods：In the in vitro and in vitro experiments，the
cells and animals were devided into the control group，lipopolysaccharide（LPS）group，and LPS + TCDCA group. In the in vitro
experiments，MTT method was firstly applied to screen the appropriate working concentration of TCDCA，and then LPS stimulation was
given to the LPS group，and LPS and TCDCA stimulation were given to the LPS + TCDCA group subsequently. The expression of
inflammation⁃related mRNAs and proteins were examined by RT⁃qPCR and Western blot，respectively . In the in vivo experiments，the
LPS group was injected with LPS solution intravenously through the ear margin of rabbits，and the LPS+TCDCA group was treated as
above and then fed with TCDCA solution through drinking water. Histopathological alterations of the samples were assessed by HE
staining，periodic acid⁃Schiff staining and Alcian blue⁃nuclear fast red staining after experimental sampling. Results：The results of in
vitro experiments showed that the expression of tumor necrosis factor⁃α，interleukin⁃1β，interleukin⁃6，and interferon⁃γ indexes was
significantly reduced and the expression of interleukin⁃10 indexes was elevated in macrophages in the LPS+TCDCA group compared
with that in the LPS group. In the in vivo experiments，the results of HE staining showed that the inflammation of intestinal tissues was
relieved，and the results of periodic acid⁃Schiff staining and Alcian blue⁃nucleic solid red staining showed that the number of cup cells

［基金项目］ 国家自然科学基金（82271003，82201096）；江苏高校“青蓝工程”中青年学术带头人项目；江苏省科教能力提升工
程——江苏省研究型医院建设单位（YJXYYJSDW4）；江苏省医学创新中心（CXZX202227）
∗
通信作者（Corresponding author），E⁃mail：qiujing@njmu.edu.cn