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第45卷第1期 王 巧，强静超，卞 乐，等. 水晶兰苷通过调节NF⁃κB/NLRP3 炎症小体通路改善脓毒症相关
2025年1月 急性肾损伤和功能障碍［J］. 南京医科大学学报（自然科学版），2025，45（1）：1-12 · 9 ·

A B C
4 3 5 ***
*** 4
***
Relative mRNA level of TNF⁃α 2 1 ### ### Relative mRNA level of IL⁃1β 2 1 ### ### Relative mRNA level of IL⁃6 3 2 ###
3

0 0 1 0 ###
CLP
Sham+MON
CLP
NC Sham CLP+MON CLP+DEX NC Sham CLP+MON CLP+DEX NC Sham CLP+MON CLP+DEX
CLP
Sham+MON
Sham+MON
D E F
1 500 400 *** 1 000
（pg/mL） 1 000 *** ### ### （pg/mL） 300 # ## （pg/mL） 800 *** ### ###
600
200
TNF⁃α 500 IL⁃1β 100 IL⁃6 400
200
0 0 0
NC Sham CLP+MON CLP+DEX NC Sham CLP+MON CLP+DEX NC Sham CLP+MON CLP+DEX
CLP
CLP
Sham+MON
Sham+MON
Sham+MON
CLP
A-C：The mRNA expression levels of TNF⁃α（A），IL⁃1β（B）and IL⁃6（C）in the kidney tissues were measured by RT⁃qPCR. D-F：ELISA was
***
used to measure the secretion levels of TNF⁃α（D），IL⁃1β（E）and IL⁃6（F）in the serum of mice. Compared with the sham group， P < 0.001；compared
##
#
with the CLP group，P < 0.05，P < 0.01，and ### P < 0.001（n=6）.
图6 MON对S⁃AKI小鼠肾组织和血清中促炎细胞因子释放的影响
Figure 6 Effects of MON on the release of pro⁃inflammatory cytokines in kidney tissues and the serum of the S⁃AKI mice
A
Sham+MON
NC Sham CLP CLP+MON CLP+DEX
NLRP3 114 kDa
Caspase⁃1 45 kDa
Cleaved⁃Caspase⁃1 20 kDa
β⁃actin 42 kDa
B C D
1.5 1.5 2.0
*** * 1.5 **
NLRP3/β⁃actin 1.0 # ### Caspase⁃1/β⁃actin 1.0 # # Cleaved⁃Caspase⁃1/ β⁃actin 1.0 #

0.5
0.5
0.0 0.0 0.5 #
0.0
CLP
Sham+MON
CLP
Sham+MON
CLP
Sham+MON
NC Sham CLP+MON CLP+DEX NC Sham CLP+MON CLP+DEX NC Sham CLP+MON CLP+DEX
E F 1.0 * G 1.5
Sham+MON
NC Sham CLP CLP+MON CLP+DEX 0.8 1.0 # ##
p⁃NF⁃κB P65 65 kDa p⁃NF⁃κB P65/β⁃actin 0.6 # # IκBα/β⁃actin
IκBα 32 kDa 0.4 0.5 **
0.2
β⁃actin 42 kDa 0.0 0.0
Sham+MON
CLP
CLP
Sham+MON
NC Sham CLP+MON CLP+DEX NC Sham CLP+MON CLP+DEX
A：Western blot was used to detect the protein expressions of NLRP3，Caspase⁃1，and Cleaved⁃Caspase⁃1 in the kidney. B-D：Quantification of NL⁃
RP3（B），Caspase⁃1（C），and Cleaved⁃Caspase⁃1（D）protein expression levels. E：Western blot was used to detect the protein expression levels of p⁃NF⁃
κB P65 and IκBα in the kidney. F，G：Quantification of p⁃NF⁃κB P65（F）and IκBα（G）protein expression. Compared with the sham group，P < 0.05，
*
** *** # ## ###
P < 0.01，and P < 0.001；compared with the CLP group，P < 0.05，P <0.01，and P < 0.001（n=3）.
图7 MON对S⁃AKI小鼠肾脏NLRP3炎性小体激活和NF⁃κB信号通路的影响
Figure 7 Effects of MON on NLRP3 inflammasome activation and NF⁃κB signaling pathway in the kidney of the S⁃AKI mice