Page 67 - 南京医科大学自然版

第45卷第10期 南京医科大学学报（自然科学版）
2025年10月 Journal of Nanjing Medical University（Natural Sciences） ·1435 ·

·基础研究·

APOE对前列腺癌细胞增殖、迁移和侵袭的影响

王 勇 ，丁 磊 ，董汇昱 ，梁 超 ，李 杰 1*
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南京医科大学第一附属医院泌尿外科，江苏 南京 210029；南京医科大学附属无锡人民医院泌尿外科，江苏 无锡 214023
［摘 要］ 目的：探讨敲降载脂蛋白E（apolipoprotein E，APOE）对前列腺癌细胞增殖、迁移、侵袭等恶化特性的影响，并通过裸
鼠在体实验进行验证。方法：通过转染小干扰RNA（small interfering RNA，siRNA）下调3种人前列腺癌细胞系LNCap、DU145和
PC⁃3中APOE的表达，通过CCK⁃8实验检测细胞活力和对抗癌药物的敏感性，克隆形成实验检测细胞增殖能力，Transwell小室
检测细胞迁移和侵袭能力，EdU实验检测前列腺癌细胞增殖情况，通过裸鼠成瘤实验验证敲降APOE及使用雄激素受体抑制
剂在体内对前列腺癌肿瘤生长的影响。结果：siRNA介导的APOE下调显著降低了LNCap、DU145和PC⁃3细胞的活性及增殖、
迁移和侵袭能力，并增加了LNCap细胞对抗癌药物的敏感度，降低了相对偏振细胞的百分比。体内实验结果同样表明，敲除
APOE显著减缓了肿瘤的生长速度，加入雄激素受体抑制剂显示出更加明显的抑癌效果。结论：APOE可调控前列腺癌细胞增
殖和转移等一系列恶性行为，调控APOE的表达可能对抑制前列腺癌的恶化进展具有重要作用。
［关键词］ 前列腺癌；载脂蛋白E；增殖；迁移；侵袭
［中图分类号］ R737.25 ［文献标志码］ A ［文章编号］ 1007⁃4368（2025）10⁃1435⁃09
doi：10.7655/NYDXBNSN250225

The effects of APOE on the proliferation，migration，and invasion of prostate cancer cells
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WANG Yong ，DING Lei ，DONG Huiyu ，LIANG Chao ，LI Jie 1*
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1 Department of Urology，the First Affiliated Hospital of Nanjing Medical University，Nanjing 210029；Department of
Urology，the Affiliated Wuxi People’s Hospital of Nanjing Medical University，Wuxi 214023，China
［Abstract］ Objective：This study explored the effects of knocking down apolipoprotein E（APOE）on the proliferation，migration，
invasion，and other malignant characteristics of prostate cancer cells through in vitro experiments，and validated them through nude
mouse in vivo experiments. Methods：The expression levels of APOE in three human prostate cancer cell lines LNCap，DU145，and
PC ⁃ 3 were downregulated by transfecting small interfering RNA（siRNA）. Cell viability and sensitivity to anticancer drugs were
detected through CCK⁃8 experiments，cell proliferation ability was detected through clone formation assay，cell migration and invasion
were detected using transwell assay，the EdU assay was used to detect the proliferation of prostate cancer cells，and the effects of
knocking down APOE and using androgen receptor inhibitor on prostate cancer tumor growth were investigated in vivo by nude mouse
tumorigenesis experiments. Results：siRNA ⁃ induced downregulation of APOE expression significantly reduced the viability，

proliferation，migration，and invasion ability of LNCap，DU145，and PC⁃3 cells，increased cell sensitivity to anticancer drugs，and
reduced the percentage of cells with relative polarization fluorescence. The results of in vivo experiments also showed that knocking
down APOE significantly slowed down the growth rate of tumors，and the addition of androgen receptor inhibitor showed a more
significant anti⁃cancer effect. Conclusion：APOE could regulate a series of malignant behaviors such as proliferation and metastasis of
prostate cancer cells，and the expression regulation of APOE may play a crucial role in inhibiting the malignancy and progression of
prostate cancer.
［Key words］ prostate cancer；apolipoprotein E；proliferation；migration；invasion
［J Nanjing Med Univ，2025，45（10）：1435⁃1442，1512］

［基金项目］ 江苏省科教能力提升工程（ZDXK202219）
通信作者（Corresponding author），E⁃mail：drc_lijie@126.com（ORCID：0000⁃0003⁃0260⁃1611）
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