Page 12 - 南京医科大学自然版

第45卷第11期
·1542 · 南 京 医 科 大 学 学 报 2025年11月

A B C
Cellular response to hypoxia p21
-lg（P） cor=0.262 expression 10
Positive regulation of angiogenesis （log2 TPM） 3 P<0.001 8
Response to hypoxia 5.0 6
4.5 2
Negative regulation of cell proliferation RBMS3 4
4.0 R =0.407
2
Activation of MAPK activity 3.5 1 Relative p21 2 P < 0.000 1
Extracellular matrix organization count Expression level 0 0
5 4 6 8 10 12 14
Positive regulation of cell migration
10 2 4 6 8 10 Relative RBMS3 expression
Cell⁃cell signaling 15 Expression level（log2 TPM）
Positive regulation of cation channel activity 20
25
Cell adhesion
2.5 3.0 3.5 4.0
mRNA expression 2.0 *** * mRNA expression 1.5 Sh2 *** RBMS3 21 kDa （/β⁃actin） 1.5 *** *
D NC E SCR F SUM⁃1315 MDA⁃MB⁃231 G SUM⁃1315⁃NC
Sh1
SUM⁃1315⁃RBMS3
NC RBMS3
NC RBMS3
RBMS3
***
p21
*
1.5
*
1.0
48 kDa
1.0
1.0
Relative p21 0.5 0 MDA⁃MB⁃231 Relative p21 0.5 0 SUM⁃1315 MDA⁃MB⁃231 β⁃actin 42 kDa Protein level 0.5 0 p21 RBMS3
SUM⁃1315
H I J
SUM⁃1315
MDA⁃MB⁃231⁃NC SCR Sh1 Sh2 SCR Sh1 Sh2 （/β⁃actin） 1.5 * * ** SUM⁃1315⁃SCR
MDA⁃MB⁃231
***
MDA⁃MB⁃231⁃RBMS3
（/β⁃actin） 2.0 ** ** RBMS3 21 kDa 1.0 SUM⁃1315⁃Sh2
SUM⁃1315⁃Sh1
p21
1.5
0.5
48 kDa
Protein level 1.0 0 β⁃actin 42 kDa Protein level 0 p21 RBMS3
0.5
K p21 RBMS3 L RBMS3 p21 Ki67
MDA⁃MB⁃231⁃SCR
MDA⁃MB⁃231⁃Sh1
MDA⁃MB⁃231⁃Sh2 SUM⁃1315⁃NC
（/β⁃actin） 1.5 * **
**
**
1.0
Protein level 0.5 0 p21 RBMS3 SUM⁃1315⁃RBMS3

A：Bubble plot of GO functional enrichment analysis based on differentially expressed genes of RBMS3. B：Scatter plot analyzing the correlation be⁃
tween RBMS3 and p21 mRNA expression in breast cancer tissues from the TIMER database. C：Scatter plot showing the relative mRNA expression
2
levels of RBMS3 and p21 in 34 paired breast cancer tissues（two⁃tailed Spearman correlation analysis，R =0.407，P < 0.000 1）. D，E：mRNA expression
levels of p21 in SUM⁃1315 and MDA⁃MB⁃231 cells after overexpression（D）or knockdown of RBMS3（E）. Relative RNA expression was calculated us⁃
ing the 2 -ΔΔCt method and normalized to β⁃actin. F-K：Protein expression levels of p21 and grayscale analysis after overexpression（F-H）or knockdown
（I-K）of RBMS3 in the two cell lines. L：Immunohistochemical staining of RBMS3，p21，and Ki⁃67 in xenograft tumor tissues from the SUM⁃1315 over⁃
* ** ***
expression group（SUM⁃1315⁃RBMS3）and the control group（SUM⁃1315⁃NC）（scale bar：50 μm）. P < 0.05，P < 0.01，and P < 0.001（n=3）.
图3 RBMS3上调乳腺癌细胞中p21的表达
Figure 3 RBMS3 upregulated p21 expression in breast cancer cells

3′⁃UTR的报告质粒后，RBMS3过表达组荧光素酶活 区域的报告质粒后，两组荧光素酶活性差异无统计
性显著高于对照组；而转染含p21 3′⁃UTR ARE突变 学意义（图4J、K）。以上结果表明，RBMS3通过结合