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A B C D
SUM⁃1315⁃NC 2.0 MDA⁃MB⁃231⁃NC Control Si⁃p21 1.5 * *
SUM⁃1315
Relative expression of p21 2.0 0 ** Relative expression of p21 1.5 0 * * β⁃actin NC RBMS3 NC RBMS3 21 kDa Protein level of p21 （/β⁃actin） 0.5 0 NC⁃Si⁃p21
MDA⁃MB⁃231⁃RBMS3
SUM⁃1315⁃RBMS3
1.0
2.5
*
1.5
1.0
p21
1.0
NC⁃Control
RBMS3⁃Si⁃p21
RBMS3⁃Control
0.5
0.5
42 kDa
E Control Si⁃p21 F Control Si⁃p21 G H
MDA⁃MB⁃231 1.5 ** SUM⁃1315 SUM⁃1315⁃NC
Control Si⁃p21 1.0 * Control Si⁃p21 SUM⁃1315⁃RBMS3
NC RBMS3 NC RBMS3 Protein level of p21 （/β⁃actin） 0.5 200 ** *
150
p21 21 kDa 0 NC Colony number 100
NC⁃Control NC⁃Si⁃p21 RBMS3 0 Control Si⁃p21
RBMS3⁃Si⁃p21
RBMS3⁃Control
β⁃actin 42 kDa 50
I J
MDA⁃MB⁃231 MDA⁃MB⁃231⁃NC
Control Si⁃p21 MDA⁃MB⁃231⁃RBMS3
K
150 Hoechst EdU Merge
Colony number 50 NC/Control
NC 100 ** **

0 Control Si⁃p21
RBMS3 RBMS3/Control

SUM⁃1315
L
Hoechst EdU Merge
NC/Control NC/Si⁃p21

RBMS3/Control RBMS3/Si⁃p21

MDA⁃MB⁃231 NC/Si⁃p21 M （%） 60 SUM⁃1315⁃NC N （%） 80 MDA⁃MB⁃231⁃NC

SUM⁃1315⁃RBMS3
MDA⁃MB⁃231⁃RBMS3
***
***
***
**
60
RBMS3/Si⁃p21 Rate of EdU positive cells 40 0 Control Si⁃p21 Rate of EdU positive cells 40 0 Control Si⁃p21
20
20

A，B：SUM⁃1315（A）and MDA⁃MB⁃231（B）cells in the RBMS3 overexpression group and the control group were transfected with si⁃p21，and
mRNA expression levels were verified by qRT⁃PCR. C-F：Western blot and densitometric analysis were used to detect protein expression after transfec⁃
tion with si⁃p21 in SUM⁃1315（C，D）and MDA⁃MB⁃231（E，F）cells. G-J：Colony formation assay was performed to assess cell proliferation ability of
SUM⁃1315（G，H）and MDA⁃MB⁃231（I，J）. K-N：EdU incorporation assay was used to detect cell proliferation after transfection with si⁃p21 in
*
**
SUM⁃1315（K，M）and MDA⁃MB⁃231（L，N）cells（scale bar：200 μm）. P < 0.05，P < 0.01，and *** P < 0.001（n=3）.
图5 p21敲低逆转了RBMS3过表达诱导的乳腺癌细胞增殖抑制和生长抑制
Figure 5 Knockdown of p21 reversed the RBMS3 overexpression⁃induced inhibition of proliferation and growth in breast
cancer cells