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第45卷第11期 林俊杰，孙君健，王 璐，等. IFN⁃γ通过抑制SRC/Areg信号轴减轻克罗恩病肠纤维化［J］.
2025年11月 南京医科大学学报（自然科学版），2025，45（11）：1608-1615，1625 ·1613 ·

A B
Control TGF⁃β TGF⁃β+IFN⁃γ IFN⁃γ *
50 *** ***
（%） 40
DAPI 30 ***
Percentage of positive cells 20

Ki67 10
Ki67 0
Control TGF⁃β+IFN⁃γ IFN⁃γ
TGF⁃β

A C 50 ** *
α⁃SMA （%） 40 *
Percentage of α⁃SMA positive cells 30

20

Merge 10 0
TGF⁃β
Control TGF⁃β+IFN⁃γ IFN⁃γ

A：Representative immunofluorescence images showing the expression of the proliferation marker Ki67（red）and the activation marker α⁃SMA
（green）in human intestinal fibroblasts treated with or without TGF⁃β and IFN⁃γ. Nuclei were counterstained with DAPI（blue，scale bar=100 μm）. B：
Quantitative analysis of the percentage of Ki67⁃positive cells. C：Quantitative analysis of the percentage of α⁃SMA positive cells. P < 0.05，P < 0.01，
*
**
and *** P < 0.001（n=3）.
图4 IFN⁃γ抑制肠成纤维细胞活化及增殖
Figure 4 IFN⁃γ inhibited activation and proliferation of intestinal fibroblasts

A B
No difference（15 913）
Up（650）
Down（795）

100
80
P 60
-lg Regulation
40
Jun
20 Down
0 Up
SRC Stat3
-10 -5 0 5 10 15 Pml Size
log2 （fold change）
25
50
Uba7 75
100
H2⁃K1 H2⁃T23
H2⁃D1 B2m

Mpeg1
Parp9
A：Transcriptomic profiling by RNA⁃seq revealed the quantity of differentially expressed genes in fibroblasts. B：Protein⁃protein interaction network
depicting genes（as nodes）and their interactions（edges）. Node size corresponds to the degree of connectivity.“Up”and“Down”indicate upregulated
and downregulated genes，respectively.
图5 IFN⁃γ可能通过抑制SRC下调Areg表达调控肠成纤维细胞活化
Figure 5 IFN⁃γ regulated the activation of intestinal fibroblasts by downregulating Areg expression through inhibiting SRC