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第45卷第4期 陈 讯，陈雯昕，张 文，等. 基于焦亡相关基因构建多囊卵巢综合征诊断模型［J］.
2025年4月 南京医科大学学报（自然科学版），2025，45（4）：509-522 ·515 ·

enrichment analysis，GSEA）进行通路富集分析。在 和免疫反应生物学功能呈正相关（图 6A）。NLRC4
GO 功能分析中，CHMP4B 与防御反应、细胞外囊泡 与防御反应、生物刺激反应、免疫反应呈负相关（图

A B C
Consensus matrix K=2
1.0
0.8 0.8
C1
C2 2 0.6
CDF 0.6 3 4 Relative change in area under CDF curve
0.4 5 0.4
6
0.2 7 0.2
8
9
0 0
0 0.2 0.4 0.6 0.8 1.0 2 3 4 5 6 7 8 9
Consesus index K

D Cluster C1 C2
* * * * * * * * * * * * * *
12.5
Gene expression 10.0

7.5
5.0

BAX1 BAX CASP1 CASP3 CASP4 CASP5 CHMP2A CHMP2B CHMP4B CHMP4C CHMP6 CHMP7 CYCS ELANE GSDMD GZMB HMGB1 IL18 IL1A IL1B IRF1 IRF2 TP53 TP63 AIM2 CASP6 CASP8 CASP9 GPX4 GSDMB GSDMC IL6 NLRC4 NLRP1 NLRP2 NLRP3 NLRP6 NLRP7 NOD1 NOD2 PLCG1 PRKACA PYCARD SCAF11 TIRAP TNF GZMS

E 3 Cluster F KEGG_PROPANOATE_METABOLISM
Cluster
BAX C1 KEGG_CELL_CYCLE
CASP4 C2 KEGG_PANTOTHENATE_AND_COA_BIOSYNTHESIS
GSDMD 2 KEGG_CYSTEINE_AND_METHIONINE_METABOLISM
CHMP2B
HMGB1 KEGG_VALINE_LEUCINE_AND_ISOLEUCINE_DEGRADATION
CASP1 KEGG_UBIQUITIN_MEDIATED_PROTEOLYSIS
SCAF11 1
IL18 KEGG_FATTY_ACID_METABOLISM
CASP5 KEGG_LYSINE_DEGRADATION
TIRAP 0
IL1A KEGG_PROSTATE_CANCER
AIM2 KEGG_MISMATCH_REPAIR
TP53 Group
GZMA -1 KEGG_P53_SIGNALING_PATHWAY
NLRP7 KEGG_RIBOSOME Down
PRKACA Up
BAK1 KEGG_TYPE_I_DIABETES_MELLITUS
GPX4 -2 KEGG_CYTOSOLC_DNA_SENSING_PATHWAY
IL1B
IL5 KEGG_HEMATOPOIETIC_CELL_LINEAGE
NLRP3 KEGG_INTESTINAI_IMMUNE_NETWORK_FOR_IGA_PRODUCTION
NLPC4 -3
NOD2 KEGG_ASTHMA
NLRP1 KEGG_AUTOIMMUNE_THYROID_DISEASE
TNF
CHMP6 KEGG_AUTOIMMUNE_THYROID_DISEASE
PYCARD KEGG_GRAFT_VERSUS_HOST_DISEASE
CHMP2A
CHMP4B KEGG_ALLOGRAFT_REJECTION
GSDMB KEGG_ANTIGEN_PROCESSING_AND_PRESENTATION
IRF1
NOD1 -10 0
PLCG1
ELANE t value of GSVA score
NLRP6
CHMP4C C2 vs. C1
TP63
CHMP7
GASP9
GSDMC
CYCS
GZMB
IRF2
CASP8
CASP6
CASP3
NLRP2
A：Consensus matrix heat map defining two clusters（K=2）and their correlation area. B：Representative CDF curves and CDF delta area curves. C：
*
The score of cluster⁃consensus. D：Boxplot showing the expression patterns of all pyroptosis⁃related genes between two clusters，P < 0.05. E：Heatmap
of expressions of all pyroptosis⁃related genes between two clusters. F：Gene set enrichment analysis（GSEA）between the two clusters.
图4 基于焦亡相关基因的新亚型鉴定
Figure 4 Identification of novel subtypes based on pyroptosis⁃related genes