Page 36 - 南京医科大学自然版

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A HE Masson B C D
（%） 30 *** 60 *** 40 *
Collagen volume fraction 10 （μmmol/L） 20 20
SYN 20 40 （mmol/L） 30

CAD 0 Scr 0 BUN 10 0

E F4/80 α⁃SMA DAPI SYN CAD Merge SYN CAD 40 *** SYN CAD
F
Relative fluoresence 20
SYN （fold of Vector） 30

intensity
10
0
SYN CAD
CAD

A：HE staining and Masson staining in transplanted kidneys of the SYN group and CAD group（×400）. B：Semi⁃qantitative analysis of collagen vol⁃
***
ume fraction in transplanted kidneys of the SYN group and CAD group（ P < 0.001，n=6）. C：Serum creatinine levels in the SYN group and CAD group
*
***
（ P < 0.001，n=6）. D：Blood urea nitrogen（BUN）levels in the SYN group and CAD group（ P < 0.05，n=6）. E：Co⁃expression of F4/80 and α⁃SMA in
transplanted kidneys from the SYN and CAD group mice by immunofluorescence staining（×400）. F：Semi⁃quantitative fluorescence intensity of co⁃ex⁃
***
pressed F4/80 and α⁃SMA in transplanted kidneys from the SYN group and CAD group（ P < 0.001，n=6）.
图1 两组小鼠移植肾纤维化水平和MMT表达
Figure 1 The degree of renal fibrosis and MMT expression in the transplanted kidneys of mice in two group

A B C
Vehicle IGT Vehicle IGT
（%）
Collagen volume fraction 40
SYN 30 *** *** SYN

20
10
CAD 0 SYN CAD CAD
CAD+IGT
SYN+IGT

D 40 *** *** E 0.08 *** *** F 0.06 *** ** G *** ***
Relative fluoresence （fold of Vector） intensity 30 Relative expression of α⁃SMA mRNA 0.06 Relative expression of ColⅠ mRNA 0.04 ns Relative expression of Fibronectin mRNA 0.08 ns
***
0.06
20
0.04
0.04
0.02
10
0.02
0.02
0
CAD
SYN
SYN+IGT CAD 0 SYN CAD+IGT 0 SYN+IGT CAD+IGT 0 SYN+IGT CAD
SYN
CAD+IGT
SYN
SYN+IGT
CAD
CAD+IGT
A：Masson staining of transplanted kidneys in different groups（×400）. B：Semi⁃qantitative analysis of collagen volume fraction in transplanted kid⁃
neys in different groups（ P < 0.001，n=5）. C：Immunofluorescence staining of F4/80 and α⁃SMA in transplanted kidneys of different groups（×400）. D：
***
***
Semi⁃quantitative fluorescence intensity of co⁃expressed F4/80 and α⁃SMA in transplanted kidneys from each group（ P < 0.001，n=5）. E：mRNA ex⁃
***
pression levels of α⁃SMA in transplanted kidneys of different groups（ P < 0.001，n=5）. F：mRNA expression levels of Col Ⅰ in transplanted kidneys of
***
**
different groups（ P < 0.01 and *** P < 0.001，n=5）. G：mRNA expression levels of Fibronectin in transplanted kidneys of different groups（ P < 0.001，
n=5）.
图2 IGT可明显减轻模型小鼠的移植肾间质纤维化进展
Figure 2 IGT significantly alleviated the progression of interstitial fibrosis in transplanted kidneys of the model mouse