Page 35 - 南京医科大学自然版

第45卷第6期 崔 闯，孙思怡，秦梓一，等. 黄芩苷激活自噬抑制炎症促进牙髓干细胞成牙/骨分化［J］.
2025年6月 南京医科大学学报（自然科学版），2025，45（6）：766-776 ·773 ·

A LPS
（100 ng/mL）+
supernatant
PMA IFN⁃γ osteogenic/odontogenic
（100 ng/mL） （20 ng/mL） BA differentiation
24 h 48 h 24 h

THP⁃1 M0 M1 M1+BA hDPSC
B C
CM⁃M0 CM⁃M1 CM⁃M1+BA CM⁃M0 CM⁃M1 CM⁃M1+BA

200 μm 200 μm 200 μm 200 μm 200 μm 200 μm

Relative mRNA expression of ALP 1.0 Relative mRNA expression of DSPP 1.0 Relative mRNA expression of RUNX⁃2 1.0 *** ***
D 1.5 *** ** 1.5 ** *** 1.5

0.5
0.5
0.5
0
CM⁃M1+BA
CM⁃M0 CM⁃M1 0 CM⁃M0 CM⁃M1 0 CM⁃M0 CM⁃M1
CM⁃M1+BA
CM⁃M1+BA
The ratio of COL⁃1/GAPDH 1.0 The ratio of DSPP/GAPDH
E 1.5 *** ** 1.5 ** *
1.0
CM⁃M1+BA
CM⁃M0 CM⁃M1 0.5 0.5

COL⁃1 139 kDa 0 0
CM⁃M1+BA
CM⁃M1+BA
DSPP 131 kDa CM⁃M0 CM⁃M1 CM⁃M0 CM⁃M1

The ratio of OPN/GAPDH The ratio of ALP/GAPDH
OPN 45 kDa 1.5 ** ** 1.5 * *

ALP 39 kDa 1.0 1.0

GAPDH 37 kDa 0.5 0.5

0
CM⁃M1+BA
CM⁃M1+BA
CM⁃M0 CM⁃M1 0 CM⁃M0 CM⁃M1
A：Schematic diagram of cell model establishment. B：The expression of ALP，DSPP，and RUNX2 in each group was detected by RT⁃PCR. C：Rep⁃
resentative images of conditioned medium treated hDPSC ALP staining on day 7. D：Representative images of alizarin red staining of hDPSC treated
**
***
*
with conditioned medium on day 21. E：The expressions of COL⁃1，DSPP，OPN and ALP were detected by Western blot. P < 0.05，P < 0.01， P <
0.001，n=3.
图5 BA处理的THP⁃1来源的M1巨噬细胞对hDPSC成牙/骨分化的影响
Figure 5 Effect of BA⁃treated THP⁃1⁃derived M1 macrophages on osteogenic/odontogenic differentiation of hDPSC