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A B
Control PARP1⁃WT PARP1⁃K192A
Control PARP1⁃WT PARP1⁃K192A

140 kDa
StrepⅡ
50 μm
GAPDH 37 kDa

50 μm
C D
0 h 24 h 48 h
Control
PARP1⁃WT
PARP1⁃K192A
Control （%） 150 ***
Initial area uncovered by migration 100 *** *** ***
***
***

50
WT
0
0 h 24 h 48 h

K192A

F 200 *
** **
Colony numbers 100
E 150
Control PARP1⁃WT PARP1⁃K192A

50

0
Control PARP1⁃K192A
PARP1⁃WT

A，B：Fluorescence imaging（A）and Western blot（B）showed that either PARP1⁃WT⁃EGFP⁃strep Ⅱ and PARP1⁃K192A⁃EGFP⁃strep Ⅱ could be
efficiently transfected into A2780 cells and expressed at a similar level（scale bar=50 μm.）. C，D：Scratch assay on A2780 cell monolayers showed that
PARP1⁃WT OE significantly decreased the closure rate of the scratched area，while PARP1⁃ K192A/Q had much less impact. E，F. Colony formation as⁃
say showed that PARP1⁃WT OE significantly decreased the number of cell colonies formed by A2780 cells，while PARP1⁃ K192A/Q had much less im⁃
*
**
pact. P < 0.05，P < 0.01 and *** P < 0.001（n=3）.
图6 PARP1过表达降低了A2780细胞的迁移和克隆形成
Figure 6 PARP1 overexpression decreased migration and colony formation in A2780 cells
Data availability statement： Conflict of Interests：
The data that support the findings of this study are avail⁃ The authors declared that they have no conflicts of interest
able from the corresponding author upon reasonable request. to this work.
Supplementary dataset 1 has been deposited into Zenodo（DOI： 作者贡献声明：
10.5281/zenodo.8164720）. 苏宁负责大部分实验操作、数据收集与分析工作；曹颖
利益冲突声明： 完成了诸多基础性工作（如临床样本采集、全蛋白翻译后修
所有作者声明无利益冲突。 饰筛选等）；张淑平参与实验操作、数据收集与分析工作；应