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A Control PARP1⁃WT PARP1⁃K192A B ** D 2.5
（A.U.） 0.8
p⁃ERK1/2 42 kDa 1.0 *** *** （A.U.） 2.0
GAPDH 37 kDa level 1.5
level 0.6
C 0.4 1.0
p⁃ERK1/2 0.5
Control PARP1⁃WT PARP1⁃K192A ERK1/2
ERK1/2 42 kDa 0.2
0 0
PARP1⁃K192A
GAPDH 37 kDa Control
Control PARP1⁃K192A PARP1⁃WT
PARP1⁃WT
A，B：Western blotting and quantification showed that PARP1 over expression significantly decreased p⁃ERK1/2. C，D：Western blotting showed
**
the protein levels of ERK1/2 in Control，PARP1⁃WT，and PARP1⁃K192A were identical，with GAPDH as an internal control. P < 0.01 and *** P < 0.001
（n=3）.
图9 PARP1过表达降低p⁃ERK1/2水平
Figure 9 PARP1 overexpression decreased p⁃ERK1/2

Author’s Contributions： cer：Mechanisms in tumour biology and therapeutic poten⁃
SU Ning was the primary charger in most of the experi⁃ tials［J］. Clin Transl Med，2024，14（11）：e70070
ments，data collection and analysis，CAO Ying did many funda⁃ ［8］ ZHANG D，TANG Z Y，HUANG H，et al. Metabolic regu⁃
mental jobs（clinical sample collection，pan⁃PTM screen，etc）； lation of gene expression by histone lactylation［J］. Na⁃
ZHANG Shuping made substantial contribution；YING Xiaoyan， ture，2019，574（7779）：575-580
YANG Lili，and ZHANG Dong designed the research；All others ［9］ JIANG J，HUANG D L，JIANG Y，et al. Lactate modu⁃
assisted in some of the experiments. ZHANG Dong wrote the lates cellular metabolism through histone lactylation⁃me⁃
manuscript with the assistance of SU Ning. YING Xiaoyan and diated gene expression in non⁃small cell lung cancer［J］.
YANG Lili proofread and gave advice. All authors read and ap⁃ Front Oncol，2021，11：647559
proved the final manuscript. ［10］LI F，ZHANG H H，HUANG Y，et al. Single⁃cell transcrip⁃
tome analysis reveals the association between histone lac⁃
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