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第46卷第10期 段学诚，孟 艳. 过氧化物还原酶4蛋白在衰老小鼠卵巢颗粒细胞中的核转位研究［J］.
2026年10月 南京医科大学学报（自然科学版），2026，46（10）：1450-1456 ·1455 ·

A 20 *** 2.5 *** *** 2.5 8 ***
relative experssion 15 *** *** *** relative experssion 2.0 * * relative experssion 2.0 * *** *** relative experssion 6 4 *** *** ***

1.5
1.5
10
1.0
1.0
IL⁃1α 5 0 IL⁃1β 0.5 0 IL⁃6 0.5 0 IL⁃8 2 0
0 50 125 250 500 0 50 125 250 500 0 50 125 250 500 0 50 125 250 500
Tg concentration（nmol/L） Tg concentration（nmol/L） Tg concentration（nmol/L） Tg concentration（nmol/L）
B Tg（nmol/L） 2.0 2.0 2.5
0 50 125 250 500 1.5 1.5 2.0
P16/GAPDH 1.0 P21/GAPDH 1.0 P53/GAPDH
P16 16 kDa 1.5
P21 21 kDa 0.5 0.5 1.0
P53 53 kDa 0.5
0 0 0
GAPDH 36 kDa 0 50 125 250 500 0 50 125 250 500 0 50 125 250 500
Concentration（nmol/L） Concentration（nmol/L） Concentration（nmol/L）
C
Control Tg （125 nmol/L） 2.5 * 2.0 * 3 **
P16 16 kDa 2.0 1.5
P16/GAPDH P21/GAPDH 1.0 P53/GAPDH
P21 21 kDa 1.5 2
P53 53 kDa 1.0 0.5 1
GAPDH 36 kDa 0.5
0 0 0
Control Tg Control Tg Control Tg
A：mRNA expression levels of SASP⁃related genes IL⁃1α，IL⁃1β，IL⁃6 and IL⁃8 after treatment with different concentrations of Tg for 24 h（n=4）.
*
***
Compared with the control group，P < 0.05， P < 0.001. B：Protein expression levels of P16，P21，and P53 after treatment with different concentrations
*
of Tg for 24 h. C：Protein expression levels of P16，P21，and P53 after treatment with 125 nmol/L Tg for 24 h（n=4）. Compared with control group，P <
**
0.05 and P < 0.01.
图3 Tg诱导的KGN细胞衰老模型的验证
Figure 3 Validation of Tg⁃induced KGN cell senescence model

Cytosol Nucleus 4 3 **
PRDX4/GAPDH 2 PRDX4/Lamin B1
Control Tg Control Tg 3 **
PRDX4 30 kDa 2
Lamin B1 66 kDa 1 1

GAPDH 36 kDa 0 0
Control Tg Control Tg
The distribution of PRDX4 in cytoplasm and nuclear components was detected by Western blot after nucleoplasm separation. Lamin B1 was the
internal reference of nuclear protein and GAPDH was the internal reference of cytoplasmic protein. Relative quantitative analysis of cytoplasmic/nuclear
**
PRDX4 was performed on the right side（n=4）. Compared with the control group，P < 0.01.
图4 衰老KGN细胞中PRDX4核转位的影响
Figure 4 Effect of PRDX4 nuclear translocation in senescent KGN cells

Author’s Contributions：
［参考文献］
Duan Xuecheng was responsible for experimental operation，
［1］ Wang B S，Han J，Elisseeff J H，et al. The senescence⁃
data analysis and manuscript writing and modification. Meng
associated secretory phenotype and its physiological and
Yan was responsible for the guidance of the research process，
pathological implications［J］. Nat Rev Mol Cell Biol，
the review and revision of the manuscript，the supervision of all
2024，25（12）：958-978
experimental work，the acquisition of research funds，and the
［2］ Wu C Q，Chen D，Stout M B，et al. Hallmarks of ovarian
important revision of the final manuscript. aging［J］. Trends Endocrinol Metab，2025，36（5）：418-439