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A hESC hAMSC

B DAPI TRA⁃1⁃60 NANOG TRA⁃1⁃60/NANOG/DAPI

DAPI SSEA4 OCT4 SSEA4/OCT4/DAPI

C Adipocytes Osteoblasts Chondrocytes

D
500 SSEA4 + 500 TRA⁃1⁃60 +
+
+
NaNOG
OCT4
Cell count 300 99.59% 300 99.69% 400 95.37% 400 99.07%
300
300
200
200
200 200
100 100
100 100
0 0 0 0
0
3
2
3
0
0
3
2
2
1
3
2
0
10 10 1 10 10 10 4 10 10 10 10 10 4 10 10 1 10 10 10 4 10 10 1 10 10 10 4
E 250 Fluorescence intensity 300
250
Cell count 250 95.41% + 200 98.51% + 200 95.81% + 200 96.67% +
CD44
CD90
CD73
CD105
200
150
150
150
100 100 100 100
50 50 50
0 0 0 0
2
2
0
0
-1
1
1
3
-1
3
0
-1
2
2
3
-1
1
1
0
3
10 10 10 10 10 10 4 10 10 10 10 10 10 4 10 10 10 10 10 10 4 10 10 10 10 10 10 4
250 250 300
CD11b 400 CD19 400 CD34 CD45 HLA⁃DR
200 1.71% 0% 0% 200 0% 1.48%
300 300 200
150 150
200 200
100 100
100
50 100 100 50
0 0 0 0 0
2
2
3
3
0
1
1
-1
-1
0
3
3
-1
0
2
-1
0
1
1
0
-1
2
1
2
3
10 10 10 10 10 10 4 10 10 10 10 10 10 4 10 10 10 10 10 10 4 10 10 10 10 10 10 4 10 10 10 10 10 10 4
A：Microscopic images represent the morphology of hESCs and hAMSCs. Scale bar：500 μm. B：Immunofluorescence staining confirming the expression
of hESC⁃specific markers. Scale bar：50 μm. C：Cultured hAMSCs could differentiate into adipocytes，osteoblasts，and chondrocytes. Scale bar：100 μm.
D：Flow cytometry analysis of pluripotency markers in hESCs. E：Flow cytometry analysis of cell⁃surface markers in the hAMSCs. Cells exhibited high
expression（>95%）of CD73，CD90，CD105，and CD44，but were negative or expressed very low levels（<2%）of CD11b，CD19，CD34，CD45，and HLA⁃DR
图1 hESC和hAMSC的鉴定
（n=3）.
Figure 1 Identification of hESC and hAMSC