Page 54 - 《南京医科大学学报》自然科学版2026年第2期

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·210 · 南 京 医 科 大 学 学 报 2026年2月

A B C
TBI
0.7 1.5
Sham 3 h 1 d 3 d 7 d 14 d *** ***
C3/α⁃Tubulin 0.5 S100A10/α⁃Tubulin
C3 115 kDa 0.6 1.0 **
S100A10 11 kDa 0.5

α⁃Tubulin 55 kDa 0.4
0.3 0
Sham 3 h 1 d 3 d 7 d 14 d Sham 3 h 1 d 3 d 7 d 14 d
TBI TBI
D E F * *
PBS LPS 2.0 PBS 1.0 * PBS
NC Dixdc1⁃sh NC Dixdc1⁃sh ** ** LPS 0.8 LPS
GFAP/α⁃Tubulin 1.0 C3/α⁃Tubulin
GFAP 50 kDa 1.5 ** 0.6
C3 115 kDa 0.4

S100A10 11 kDa 0.5 0.2
0 0
α⁃Tubulin 55 kDa NC NC NC NC
Dixdc1⁃sh Dixdc1⁃sh Dixdc1⁃sh Dixdc1⁃sh

G * I PBS LPS
2.0 ** PBS NC Dixdc1⁃sh NC Dixdc1⁃sh
LPS
S100A10/α⁃Tubulin 1.5 * C3

1.0
0.5

0 S100A10
Dixdc1⁃sh Dixdc1⁃sh
NC NC

H PBS LPS J PBS LPS K PBS
Dixdc1⁃sh Dixdc1⁃sh NC Dixdc1⁃sh NC Dixdc1⁃sh LPS *** *
NC NC p⁃STAT3 86 kDa 1.0
2.5 79 kDa 0.8
Serping 1 2.0 *
1.5 STAT3 79 kDa 0.6
specific H2.D1 1.0 p⁃STAT3/STAT3 0.4

A1 Gbp2 0.2

Srgn 0
NC
NC
S100A10 Dixdc1⁃sh Dixdc1⁃sh
specific Clcf1
A2
Emp1

A-C：Expression levels of C3（B）and S100A10（C）were detected by Western blot（A）. D-G：Expression levels of GFAP（E），C3（F），and S100A10
（G）were detected by Western blot（D）in astrocytes. H：mRNA expression levels of A1 and A2 phenotype⁃related genes by RT⁃PCR. I：Expression levels
of C3 and S100A10 in astrocytes were stained with immunofluorescence（C3：red/Hoechst：blue and S100A10：red/Hoechst：blue；×200）. J，K：Expression
**
*
levels of STAT3 were detected by Western blot after LPS（1 μg/mL，24 h）treatment. P < 0.05，P < 0.01 and *** P < 0.001（n=3）.
图6 敲低Dixdc1抑制LPS诱导的A1星形胶质细胞极化
Figure 6 Knockdown of Dixdc1 inhibits LPS⁃induced A1 astrocyte polarization