Page 37 - 南京医科大学自然版

第46卷第5期 王心雅，罗 琳，高 乾，等. PTPN6在胃癌中的作用及对NK细胞介导杀伤活性的影响［J］.
2026年5月 南京医科大学学报（自然科学版），2026，46（5）：652-663，690 ·659 ·

A AGS MKN1 C AGS D AGS MKN1
PTPN6 600 *** 4 000 *** 2.5 NC *** *** NC PTPN6⁃OE NC PTPN6⁃OE
PTPN6⁃OE
2.0
Relative expression of 2 1 2 000 2 1 Normalized cell index 1.0 0 1 *** 3 4 250 * 250 *
3 000
1.5
400
0.5
0.0
200
200
2
0
PTPN6⁃OE 0 PTPN6⁃OE 3 Time（days） Colony number 150 Colony number 150
NC
NC
100
MKN1
100
B NC *** 50 50
PTPN6⁃OE
AGS MKN1 2 *** 0 0
PTPN6⁃OE PTPN6⁃OE Normalized cell index *** PTPN6⁃OE PTPN6⁃OE
NC
NC
NC NC 1 ***
PTPN6 68 kDa
β⁃actin 42 kDa 0 0 1 2 3 4
Time（days）
E F
NC PTPN6⁃OE AGS
NC PTPN6⁃OE
NC
PTPN6⁃OE
DAPI Migration Cell number perfield 2 000 *** ***
1 500
1 000
Invasion 500 0

EdU Migration Invasion

E G
MKN1
NC PTPN6⁃OE NC
Merge 1 500 PTPN6⁃OE
Migration 1 000 ** **

AGS Cell number perfield 500
Relative EdU positive cells 0.3 Invasion 0 Migration Invasion
0.4
*

0.2
0.1
0
PTPN6⁃OE
NC

A：The mRNA levels of PTPN6 in AGS and MKN1 cells were detected by qRT⁃PCR（n=4）. B：The protein levels of PTPN6 in AGS and MKN1
cells were determined by Western blot. C：The levels of cell proliferation in AGS and MKN1 cells were detected by CCK⁃8 assay（n=4）. D：The levels of
cell proliferation in AGS and MKN1 cells were detected by colony formation assay. Images were from one well of a six⁃well plate（n=3）. E：The level of
cell proliferation in AGS cells was detected by EdU assay（scale bar=50 μm n=3）. F，G：The cell migration and invasion abilities of MKN1 cells（F）
**
*
and AGS cells（G）were assessed by Transwell assay（scale bar=200 μm n=3）. P < 0.05，P < 0.01，and *** P < 0.001.
图3 过表达PTPN6基因促进AGS和MKN1细胞的增殖和侵袭能力
Figure 3 PTPN6 overexpression enhanced proliferative and invasive capacity in AGS and MKN1 cells