Page 48 - 南京医科大学自然版

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·670 · 南 京 医 科 大 学 学 报 2026年5月

A 120 ### B 1.5 ### Control
tRF⁃AsnGTT⁃33/U6 100 *** Relative mRNA expression 1.0 *** ### *** ADR+tRF NC
110
ADR
90
80
ADR+tRF mimics
2.0
0.5
1.5
1.0
0.5
0
ADR+tRF mimics
ADR
Control ADR+tRF NC 0 Ncphrin Podocin
ADR+tRF mimics
C D
Control ADR ADR+tRF NC 1.5 ### Control
ADR
Nephrin 110 kDa Relative protein level 1.0 ### ADR+tRF NC
ADR+tRF mimics
Podocin 42 kDa 0.5 *** ***
GAPDH 37 kDa 0
Ncphrin Podocin
A：Expression levels of tRF⁃AsnGTT⁃33 in podocytes across experimental groups. B：Relative mRNA expression levels of Nephrin and Podocin in
podocytes from different groups. C，D：Representative Western blot bands and relative protein expression levels of Nephrin and Podocin in podocytes
***
###
among groups. Compared with the control group， P < 0.001；compared with the ADR + tRF NC group， P < 0.001（n=3）.
图4 过表达tRF⁃AsnGTT⁃33对ADR诱导足细胞损伤的影响
Figure 4 Effect of tRF⁃AsnGTT⁃33 overexpression on ADR⁃induced podocyte injury

Relative luciferase activity 1.0 Relative RAB21 expression 1.0 *** ### Relative RAB21 expression 1.0 *** ###
A 1.5 B mRNA 1.5 C mRNA 1.5

0.5
0.5
0.5
0
MUT+tRF mimics
ADR+tRF AAV
ADR
ADR
WT+tRF mimic
ADR+tRF mimics
WT+tRF NC MUT+tRF NC 0 Control ADR+tRF NC 0 Control ADR+tRF NC
ADR+tRF mimics
D E
Control ADR ADR+tRF NC ADR+tRF AAV Control ADR ADR+tRF NC
RAB21 24 kDa RAB21 24 kDa
GAPDH 37 kDa GAPDH 37 kDa
F 2.0 *** G 2.5 ***
Relative protein level of RAB21 1.5 ### Relative protein level of RAB21 2.0 ###

1.5
1.0
1.0
0.5
0.5
0
ADR
ADR+tRF mimics
ADR
ADR+tRF AAV
Control ADR+tRF NC 0 Control ADR+tRF NC
A：Luciferase reporter assay. B：Relative mRNA expression levels of RAB21 in podocytes from each group in vivo. C：Relative RAB21 mRNA ex⁃
pression levels in podocytes from each group in vitro. D：Representative Western blot bands of RAB21 protein in podocytes from each group in vivo. E：
Representative Western blot bands of RAB21 protein in podocytes from each group in vitro. F：Relative protein expression levels of RAB21 in podocytes
***
from each group in vitro. G：Relative protein expression levels of RAB21 in podocytes from each group in vivo. Compared with the control group， P <
###
0.001；compared with the ADR+tRF NC group， P < 0.001（n=3）.
图5 tRF⁃AsnGTT⁃33对RAB21表达的影响
Figure 5 Effect of tRF⁃AsnGTT⁃33 on RAB21 expression