Page 43 - 南京医科大学自然版

第46卷第6期 朱天帆，陈 峰，黄惠结. SMOC2促进低氧诱导的肺血管平滑肌细胞表型转换［J］.
2026年6月 南京医科大学学报（自然科学版），2026，46（6）：820-831 ·825 ·

A B P<0.001 NC P<0.001 NC
P<0.001 siS1 P<0.001 siS1
2.0 ▲ siS2 2.0 ▲ siS2
P<0.001
P<0.001
P<0.001 P<0.001
Normoxia Hypoxia 1.5 1.5
SMOC2/β⁃actin ▲ ▲
NC siS1 siS2 NC siS1 siS2 1.0 P<0.001 PCNA/Tubulin 1.0 P<0.001 ▲ ▲ ▲
▲
SMOC2 -54 kDa ▲ ▲ ▲ ▲
▲
β⁃actin -43 kDa 0.5 0.5
0 0
Normoxia Hypoxia Normoxia Hypoxia
PCNA -36 kDa
P<0.001
Tubulin -55 kDa NC NC
P<0.001
siS1 P<0.001 siS1
α⁃SMA -42 kDa 2.5 P<0.001 ▲ siS2 2.5 ▲ siS2
P<0.001
P<0.001
Tubulin -55 kDa 2.0 ▲ ▲ P=0.001 2.0 P<0.001 ▲ P<0.001
αSMA/Tubulin ▲ ▲ Calponin/Tubulin ■
■
Calponin1 -32 kDa 1.5 ▲ P=0.005 ▲ 1.5 ■ ■ ▲ ▲ ▲ ■ ■ ▲ ▲ ▲
Tubulin -55 kDa 1.0 1.0
0.5 0.5
0 0
Normoxia Hypoxia Normoxia Hypoxia
C
Normoxia Hypoxia
NC siS1 siS2 NC siS1 siS2

h
0

h
24
1 000 μm

D P<0.001 E F
NC P<0.001
NC siS1 siS2
siS1 P<0.001 NC
P<0.001
▲ siS2 40 P<0.001 30 P<0.001 ▲ siS2
siS1
P<0.001
（%） 30 P<0.001 ■ ■ ■ ▲ ▲ Normoxia （%） 20 P<0.001 ■ ■ ▲ ▲
P=0.003
Migration rate 20 ■ ■ ■ ▲ ▲ ▲ ▲ Hypoxia EdU positive cells 10 ■ ■ ■ ■ ■ ■ ▲ ▲ ▲ ▲ ▲▲ ■ ■ ▲ ▲ ▲ ▲
■ ■
■
10
0 400 μm 0
Normoxia Hypoxia Normoxia Hypoxia
A，B：Representative immunoblots（A）and densitometric analysis（B）of SMOC2，Calponin1，α⁃SMA and PCNA protein levels，normalized to
β⁃actin or Tubulin（n=3）. C：Representative images of the wound⁃healing assays showing migration of PASMCs. Scale bar=1 000 μm. D：Quantification
of the relative wound closure area. E：Representative fluorescence microscopic images showing EdU⁃positive cells（red）with nuclei counterstained with
DAPI（blue）. Scale bar=400 μm. F：Quantitative analysis of EdU incorporation into PASMC. At least 5 randomly chosen fields were evaluated. For all
experiments，PASMCs were transfected with SMOC2 siRNA by RNAiMax for 48 h before exposed to normoxia or hypoxia for 24 h，siS1：SMOC2⁃siRNA⁃1；
siS2：SMOC2⁃siRNA⁃2，NC：negative control.
图2 敲低SMOC2抑制PASMC迁移和增殖
Figure 2 Knockdown of SMOC2 inhibited hypoxia⁃induced proliferation and migrationof PASMC

2.3 SMOC2 重组蛋白促进 PASMC 的增殖、迁移及 利用人源 SMOC2 重组蛋白处理 PASMC。划痕实验
表型转换 （图3A、B）和EdU实验（图3C、D）的结果显示，SMOC2
为了进一步验证SMOC2调控PASMC表型转换， 重组蛋白可显著促进PASMC迁移和增殖。Western