Page 44 - 南京医科大学自然版

第46卷第6期
·826 · 南 京 医 科 大 学 学 报 2026年6月

blot 分析显示，PASMC 中增殖标志物 PCNA 蛋白表 的实验结果显示，SMOC2 是 PASMC 由静息收缩表
达增加，收缩表型标志物表达减少（图3E、F）。以上 型向缺氧刺激合成表型转换的关键正性调节因子。
A Normoxia Hypoxia
rhSMOC2 0 ng 100 ng 200 ng 500 ng 0 ng 100 ng 200 ng 500 ng

1 000 μm
B C D ▲ rhSMOC2 500 ng
▲ rhSMOC2 200 ng P<0.001
rhSMOC2 500 ng rhSMOC2（ng）
▲
■ rhSMOC2 100 ng
▲ rhSMOC2 200 ng P<0.001 P<0.001
0 100 200 500 ● NC
■ rhSMOC2 100 ng
P<0.001 P<0.001
● NC 50 P<0.001
P=0.017
50 P<0.001 Normoxia （%） 40 P<0.001 ▲ ▲ ▲ ▲ ▲
（%） 40 P<0.001 ▲ ▲ ▲ 30 P<0.001 ▲ ▲ ▲ ▲ ▲ ▲ ▲ ▲
P<0.001
P<0.001
▲
▲
migration rate 20 ▲ ▲ ▲ Hypoxia 400 μm EdU positive cells 20 ▲ ▲
30 P=0.004
▲ ▲
▲
10
10
0
0 Normoxia Hypoxia
Normoxia Hypoxia ▲ rhSMOC2 500 ng
F
▲ rhSMOC2 200 ng ▲ rhSMOC2 500 ng
■ rhSMOC2 100 ng ▲ rhSMOC2 200 ng
E
● NC ■ rhSMOC2 100 ng
P<0.001 ● NC
Normoxia Hypoxia P<0.001
rhSMOC2（ng） 0 100 200 500 0 100 200 500 P<0.001 P<0.001
2.5 P<0.001 P<0.001 3.0 P<0.001
SMOC2 -54 kDa ▲
P<0.001 P<0.001 P<0.001 P<0.001 ▲
2.0 ▲ ▲ ▲ ▲ ▲
β⁃actin -43 kDa P<0.001 ▲ ▲ ▲ ▲ ▲ ▲ 2.0 P<0.001 ▲ ▲
PCNA/Tubulin SMOC2/β⁃actin ▲ ▲ ▲
PCNA -36 kDa 1.5 ▲ ▲ ▲ P<0.001 ▲ ▲ ▲
Tubulin -55 kDa 1.0 1.0
α⁃SMA -42 kDa 0.5
0 0
Tubulin -55 kDa
Normoxia Hypoxia Normoxia Hypoxia
Calponin1 -32 kDa
rhSMOC2 500 ng ▲ rhSMOC2 500 ng
▲
▲ rhSMOC2 200 ng ▲ rhSMOC2 200 ng
Tubulin -55 kDa P<0.001 ■ rhSMOC2 100 ng P<0.001 ■ rhSMOC2 100 ng
P<0.001 ● NC ● NC
P<0.001
P<0.001 P<0.001 P<0.001
P<0.001
1.2 P<0.001 1.2 P<0.001 P<0.001
P<0.001 0.9 P<0.001
0.9
αSMA/Tubulin 0.6 ▲ ▲ ▲ ▲ ▲ ▲ ▲ ▲ ▲ ▲ ▲ Calponin/Tubulin 0.6 ▲ ▲ ▲ ▲ ▲ ▲ ▲ ▲ ▲ ▲ ▲ ▲
P<0.001
▲
0.3
0.3
0 0
Normoxia Hypoxia Normoxia Hypoxia
A，B：Representative images（A）and quantification（B）of wound⁃healing assays in PASMCs. Scale bar=1 000 μm. C，D：Representative images（C）and
quantification（D）of EdU（red）staining in PASMCs treat with or without recombinant human SMOC2（rhSMOC2），followed by hypoxia or normoxia expo⁃
sure. Scale bar=400 μm. E，F：Representative immunoblots（E）and relative densitometric analysis（F）of SMOC2，PCNA，Calponin1，and α⁃SMA protein
expression levels in PASMCs（n=3）. For all experiments，PASMCs were treated with rhSMOC2 for 24 h before exposed to normoxia or hypoxia for 24 h.
图3 SMOC2重组蛋白促进PASMC迁移和增殖
Figure 3 Recombinant SMOC2 protein promoted hypoxia⁃induced proliferation and migration of PASMCs