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·1434 · 南 京 医 科 大 学 学 报 2021年10月
表1 构建sgRNA表达载体所用引物
Table 1 Sequences of primers for constructing sgRNA expression vectors
名称 序列（5′-3′）
01THRA atgCGTCTCaACCGGAGGTTCTTCTGGATTGTGgttttagagctagaaatagcaag
02THRA atgCGTCTCaACCGCTGTCATATTTGCAGGAATgttttagagctagaaatagcaag
03THRB atgCGTCTCaACCGCTGTAAGGCCATTTTCTAAgttttagagctagaaatagcaag
04THRB atgCGTCTCaACCGAATATTGAGCTAGTCCAAGgttttagagctagaaatagcaag
05PPARa atgCGTCTCaACCGACCACAGGATAAGTCACCGgttttagagctagaaatagcaag
06PPARa atgCGTCTCaACCGACTCCGTAATGATAGCCTGgttttagagctagaaatagcaag
07PPARb atgCGTCTCaACCGGTGACAAAGCCACTGCCGTgttttagagctagaaatagcaag
08PPARb atgCGTCTCaACCGTCACTGTCATCAAGTTCCAgttttagagctagaaatagcaag
09PPARg atgCGTCTCaACCGCAGCCCTGAAAGATGCGGAgttttagagctagaaatagcaag
10PPARg atgCGTCTCaACCGGCTCCATAAAGTCACCAAAgttttagagctagaaatagcaag
11RARa atgCGTCTCaACCGCTCTCAGAGCACTCGGGCTgttttagagctagaaatagcaag
12RARa atgCGTCTCaACCGTATTTGCCCAGCTGGCAGAgttttagagctagaaatagcaag
13RARb atgCGTCTCaACCGTTCACTGAATTTGTCCCAGgttttagagctagaaatagcaag
14RARb atgCGTCTCaACCGATCTTAATAATGCACTTGGgttttagagctagaaatagcaag
15RARg atgCGTCTCaACCGGGCACCATCTCCTCTGAGCgttttagagctagaaatagcaag
16RARg atgCGTCTCaACCGCAGTACTGGCAGCGATTCCgttttagagctagaaatagcaag
17GR atgCGTCTCaACCGCTTACATCTGGTCTCATGCgttttagagctagaaatagcaag
18GR atgCGTCTCaACCGAGCACACCAGGCAGAGTTTgttttagagctagaaatagcaag
19MR atgCGTCTCaACCGTCAGACATAAATGATCTCAgttttagagctagaaatagcaag
20MR atgCGTCTCaACCGACACAGAGTTGATTCCAGCgttttagagctagaaatagcaag
21NR1H3 atgCGTCTCaACCGCGCTCCCTTGATGACGCTGgttttagagctagaaatagcaag
22NR1H3 atgCGTCTCaACCGACGCATGTAGGTGTCCATGgttttagagctagaaatagcaag
23NR1H2 atgCGTCTCaACCGTTTCCGAATCTTCTTCTTCgttttagagctagaaatagcaag
24NR1H2 atgCGTCTCaACCGTTCTTGAGCCGCTGTTAGCgttttagagctagaaatagcaag
25NR1H4 atgCGTCTCaACCGCGAGTATGGTTCCACTTCCgttttagagctagaaatagcaag
26NR1H4 atgCGTCTCaACCGTTGGAATAATAGGATGACGgttttagagctagaaatagcaag
27HNF4a atgCGTCTCaACCGCTGTTGGGCGCGTTGAGGTgttttagagctagaaatagcaag
28HNF4a atgCGTCTCaACCGGTTCTTCCGCACGCTCCTCgttttagagctagaaatagcaag
29HNF4g atgCGTCTCaACCGTGGTATTCATACTTGTCTCgttttagagctagaaatagcaag
30HNF4g atgCGTCTCaACCGCCATCAAATGTGCTTCTTCgttttagagctagaaatagcaag
31VDR atgCGTCTCaACCGATGCGGCAGTCCCCGTTGAgttttagagctagaaatagcaag
32VDR atgCGTCTCaACCGCAGTGGCGTCGGTTGTCCTgttttagagctagaaatagcaag
33RXRa atgCGTCTCaACCGATGTCCTCGCTGCTGCTGAgttttagagctagaaatagcaag
34RXRa atgCGTCTCaACCGAAGGAAGCCATGTTTCCTGgttttagagctagaaatagcaag
35RXRb atgCGTCTCaACCGGAGGAGCTGTCTGGGCTTCgttttagagctagaaatagcaag
36RXRb atgCGTCTCaACCGGAGGGACTCCCTGGGGAAGgttttagagctagaaatagcaag
37RXRg atgCGTCTCaACCGACTCGATATGGAGAGCCCAgttttagagctagaaatagcaag
38RXRg atgCGTCTCaACCGCAACCAAGTTGATTCCTGGgttttagagctagaaatagcaag
39NR2C1 atgCGTCTCaACCGATCAAGTGCTGTCACAATCgttttagagctagaaatagcaag
40NR2C1 atgCGTCTCaACCGTTGTAAGGAAAACTTTTCCgttttagagctagaaatagcaag
41NR2C2 atgCGTCTCaACCGTCAGGATGAACTGCTGTTTgttttagagctagaaatagcaag
42NR2C2 atgCGTCTCaACCGAATATGAGTTGCTTGGCGCgttttagagctagaaatagcaag
43PGC atgCGTCTCaACCGCCAAACAGGCACCAAGAGCgttttagagctagaaatagcaag
44PGC atgCGTCTCaACCGCTGTCTCCAACCTTGCACCgttttagagctagaaatagcaag
45AR atgCGTCTCaACCGTCCCTGGCAGTCTCCAAACgttttagagctagaaatagcaag
46AR atgCGTCTCaACCGGTGGAAAGTAATAGTCAATgttttagagctagaaatagcaag
47NR4A1 atgCGTCTCaACCGGCTGAGGACGAGGATGTGGgttttagagctagaaatagcaag
48NR4A1 atgCGTCTCaACCGTCAGAGCCACTGGAGGACAgttttagagctagaaatagcaag
49NR4A2 atgCGTCTCaACCGGCATTTGGTACAAGCAAGGgttttagagctagaaatagcaag
50NR4A2 atgCGTCTCaACCGCGGCATCATCTCCTCAGACgttttagagctagaaatagcaag
51NR4A3 atgCGTCTCaACCGTGGTGATGGTGATGGTAGCgttttagagctagaaatagcaag
52NR4A3 atgCGTCTCaACCGGACTGCTTGAAGTACATGGgttttagagctagaaatagcaag
53NR5A1 atgCGTCTCaACCGATCTTGCAGCTCTGGCTCTgttttagagctagaaatagcaag
54NR5A1 atgCGTCTCaACCGCATTTCTGGAAGCGGCAGAgttttagagctagaaatagcaag
55NR5A2 atgCGTCTCaACCGACACTTTATCTCCACACACgttttagagctagaaatagcaag
56NR5A2 atgCGTCTCaACCGCAAACCTTGCAGCTTTCACgttttagagctagaaatagcaag
57GCNF atgCGTCTCaACCGGTATATATTGGTAATGTGGgttttagagctagaaatagcaag
