Page 38 - 南京医科大学学报自然科学版

南京医科大学学报（自然科学版） 第42卷第2期
·184 · Journal of Nanjing Medical University（Natural Sciences） 2022年2月

·临床医学·

MCT1在预测肝细胞癌患者预后中的作用研究

韩 晟，周锦仁，邵 青，戴新征，古 鉴 *
南京医科大学第一附属医院肝胆中心，中国医学科学院肝移植重点实验室，江苏 南京 210029

［摘 要］ 目的：探讨乳酸转运载体单羧酸转运蛋白1（monocarboxylate transporter 1，MCT1）在肝细胞肝癌（hepatocellular car⁃
cinoma，HCC）患者预后预测中的应用价值及相应的调控机制。方法：筛选临床HCC患者行肝切除后的组织标本，通过比较与
癌旁组织的表达差异区分MCT1高表达组与低表达组；研究不同组患者外周血免疫细胞分型与活化情况，以及肿瘤免疫浸润
情况。回顾性分析43例HCC手术患者乳酸和MCT1高表达与低表达组的生存与复发差异。结果：MCT1高表达组与低表达组
相比，外周血免疫细胞分型无明显差异，但CD4 T细胞更易在乳酸刺激下转化为Treg（调节型T）细胞；乳酸环境下，MCT1高表
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达组Treg细胞具有明显升高的代谢水平；病理标本分析发现MCT1高表达组具有较低的炎性细胞浸润和较高的免疫抑制因子
转录水平（Foxp3、CTLA4、IL⁃10）。回顾较早手术患者分析后显示，肿瘤内乳酸表达水平与患者预后无明显相关性，而MCT1高
表达组预后和复发水平明显差于MCT1低表达组。结论：MCT1表达水平对HCC患者具备很好的预测价值，MCT1⁃Treg相关通
路可能作为新的肝癌诊断、治疗、预后评估靶点。
［关键词］ 单羧酸转运蛋白1；肝细胞癌；调节型T细胞
［中图分类号］ R735.7 ［文献标志码］ A ［文章编号］ 1007⁃4368（2022）02⁃184⁃06
doi：10.7655/NYDXBNS20220206

The role of MCT1 in predicting the prognosis of patients with hepatocellular carcinoma

HAN Sheng，ZHOU Jinren，SHAO Qing，DAI Xinzheng，GU Jian *
Hepatobiliary Center，the First Affiliated Hospital of Nanjing Medical University，Key Laboratory of Liver
Transplantation，Chinese Academy of Medical Science，Nanjing 210029，China

［Abstract］ Objective：This study aims to explore the application value and possible mechanism of monocarboxy transporter 1
（MCT1）in the prediction of prognosis for the patients with hepatocellular carcinoma（HCC）. Methods：We selected the tissues of HCC
patients after liver resection，and used RT⁃PCR to estimate the expression of MCT1 and divided the patients into MCT1 high and MCT1
low groups；the phenotype and activation of the immune cells from the peripheral blood as well as the immune statues of the resected
liver tissues from both groups were analyzed. A retrospective analysis of the relationship between the overall survival（OS）and disease⁃
free survival（DFS）rate to the expression of lactate or MCT1 between 43 HCC patients who underwent liver surgery was also compared.
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Results：Although there was no difference in the phenotype of immune cells from peripheral blood，CD4 T cells were easier to
transform into Treg cells under the stimulation of lactic acid in the high MCT1 expression group；under the environment of lactic acid，
the Treg cells from the MCT1 high expression group presented a significant increase of metabolism level；MCT1 high expression group
had lower immune cell infiltration and up⁃regulated expression of anti⁃inflammatory cytokines（Foxp3，CTLA3，IL⁃10）. The OS and
DFS rate were much worse in MCT1 high patients comparing with MCT1 low group. Conclusion：MCT1⁃Treg related targets may serve
as a new target for the diagnosis，therapeutic and prognosis prediction of HCC.
［Key words］ MCT1；HCC；Treg
［J Nanjing Med Univ，2022，42（02）：184⁃188，210］

肝细胞肝癌（hepatocellular carcinoma，HCC）是

［基金项目］ 江苏省自然科学基金（BK0201486） 最常见的恶性肿瘤。除了手术切除和肝移植外，针对
［1］
∗ HCC的抗肿瘤药物治疗效果有限，患者预后较差 。
通信作者（Corresponding author），E⁃mail：Gujian@njmu.edu.cn