Page 43 - 南京医科大学学报自然科学版

第42卷第3期 南京医科大学学报（自然科学版）
2022年3月 Journal of Nanjing Medical University（Natural Sciences） ·345 ·

·临床研究·

KLF2在急性冠脉综合征中的作用研究

陈 亮，柏 洁，郑扣龙 *
南通大学第二附属医院心血管内科，江苏 南通 226001

［摘 要］ 目的：本研究旨在探讨Kruppel样转录因子2（Kruppel⁃like factor 2，KLF2）是否能调节炎性反应在急性冠脉综合征
（acute coronary syndrome，ACS）中的作用，为今后防治提供靶点。方法：qRT⁃PCR 和 Western blot 技术检测对照组 45 例和 ACS
组 123 例外周血单个核细胞中 KLF2 mRNA 及蛋白表达水平；流式细胞术检测 ACS 组外周 T 细胞表面分子的表达水平；计算
ACS患者Gensini评分，并分析ACS组中的KLF2与Gensini评分的相关性。结果：ACS组外周血中KLF2 mRNA及蛋白表达水平

明显低于对照组；ACS组外周T细胞表面CD62L和CCR7低于对照组，而CXCR3和CD44的表达水平则明显高于对照组；KLF2
与Gensini 评分呈负相关。结论：KLF2基因在ACS 患者中低表达，可能通过调控T细胞表面CD62L、CCR7、CXCR3和CD44等
水平参于ACS的炎症反应，为ACS的防治提供新思路。
［关键词］ KLF2；ACS；炎症反应

［中图分类号］ R541.4 ［文献标志码］ A ［文章编号］ 1007⁃4368（2022）03⁃345⁃07
doi：10.7655/NYDXBNS20220306

The role of KLF2 in acute coronary syndrome

CHEN Liang，BAI Jie，ZHENG Koulong *
Department of Cardiovascular Medicine，the Second Affiliated Hospital of Nantong University，Nantong 226001，
China

［Abstract］ Objective：This study was designed to investigate whether Kruppel⁃like factor 2（KLF2）can play an important role in
acute coronary syndrome（ACS）by regulating inflammatory response，providing a potential treatment for myocardial infarction in the
future. Methods：The expressions of KLF2 mRNA and protein in peripheral blood mononuclear cells of ACS group（n=123）and control
group（n=45）were determined by quantitative real time PCR and Western blot. Flow cytometry was used to detect the expressions of
molecules on peripheral T cells of ACS group. The Gensini score of ACS patients were calculated，and the correlation between KLF2
and Gensini score in ACS patients was analyzed. Results：The expression of KLF2 protein and mRNA levels of peripheral blood
mononuclear cells in ACS group were significantly lower than those in the control group. The expressions of CD62L and CCR7 on
peripheral T cells in ACS group were lower than those in control group，while the expression levels of CXCR3 and CD44 were
significantly higher than those in control group，and KLF2 was negatively correlated with Gensini score. Conclusion：KLF2 gene is
significantly underexpressed in ACS patients，and may participate in the inflammatory response of ACS by regulating the levels of
CD62L，CCR7，CXCR3 and CD44 on the surface of T cells，which may provide a new idea for the treatment of ACS.
［Key words］ KLF2；ACS；inflammatory responses
［J Nanjing Med Univ，2022，42（03）：345⁃351］

急性冠状动脉综合征（acute coronary syndrome， 完全或不完全闭塞性血栓形成为病理基础的一组
ACS）是以冠状动脉粥样硬化斑块破裂或侵袭，继发 临床综合征。包括急性 ST 段抬高性心肌梗死
（acute ST segment elevation myocardial infarction，

［基金项目］ 江苏省卫生健康委员会项目（Z2021005）；南通 STEMI）、急性非 ST 段抬高性心肌梗死（non ST seg⁃
市科学技术局项目（MS12020016） ment elevation myocardial infarction，NSTEMI）和不稳
∗ 定型心绞痛（unstable angina，UA）。该组疾病发病
通信作者（Corresponding author），E⁃mail：zklsir@126.com