Page 44 - 南京医科大学学报自然科学版

南京医科大学学报（自然科学版） 第42卷第6期
·796 · Journal of Nanjing Medical University（Natural Sciences） 2022年6月

·基础研究·

CHL1在支气管哮喘中的表达及意义

朱 敏，王红玉，陆琳娟，姚 欣 *
南京医科大学第一附属医院呼吸与危重症医学科，江苏 南京 210029

［摘 要］ 目的：探讨神经细胞黏附分子L1样蛋白（cell adhesion molecule L1⁃like protein，CHL1）在支气管哮喘中的表达及潜
在机制。方法：收集55例哮喘患者及同期18例健康对照的临床资料及血清标本，酶联免疫吸附法检测血清CHL1水平，分析
其与肺功能指标、血清总免疫球蛋白E（immunoglobulin E，IgE）的相关性。构建卵清蛋白（ovalbumin，OVA）诱导的慢性过敏性
哮喘小鼠模型，免疫组化观察小鼠肺组织CHL1的表达定位及分布。采用不同细胞因子刺激人支气管上皮细胞（16HBE），分
别用实时定量PCR法和蛋白质免疫印迹法检测细胞CHL1 mRNA和蛋白表达情况。结果：与对照组（4.174±2.122）ng/mL相比，
哮喘患者血清CHL1水平明显增高［（7.497±3.274）ng/mL，P < 0.000 1］，与血清总IgE水平呈正相关（r=0.287，P=0.048）。CHL1
主要表达于小鼠肺组织支气管上皮细胞，哮喘小鼠表达量较对照组升高。TGF⁃β可上调支气管上皮细胞CHL1 mRNA及蛋白
表达水平。结论：哮喘中支气管上皮细胞CHL1表达增加，该过程可能与TGF⁃β相关信号通路有关。
［关键词］ 支气管哮喘；CHL1；支气管上皮细胞；TGF⁃β
［中图分类号］ R562.25 ［文献标志码］ A ［文章编号］ 1007⁃4368（2022）06⁃796⁃06
doi：10.7655/NYDXBNS20220605

The expression and significance of CHL1 in asthma

ZHU Min，WANG Hongyu，LU Linjuan，YAO Xin *
Department of Respiratory and Critical Care Medicine，the First Affiliated Hospital of Nanjing Medical University，
Nanjing 210029，China

［Abstract］ Objective：This study aims to explore the expression and potential mechanism of cell adhesion molecule L1⁃like protein
（CHL1）in bronchial asthma. Methods：The clinical data and serum samples of 55 asthma patients and 18 healthy controls were
collected，enzyme⁃linked immunosorbent assay was used to detect the expression of serum CHL1，then the correlation of CHL1 with
pulmonary function related⁃indexor serum total immunoglobulin E（IgE）was analyzed. A mouse model of ovalbumin（OVA）⁃induced
chronic allergic asthma was constructed，using immunohistochemistry to observe the localization and distribution pattern of CHL1 in
lung tissue. The expression of CHL1 after stimulated by different cytokines in human bronchial epithelial cell line 16HBE was detected
by real⁃time quantitative PCR and Western blot. Results：The level of serum CHL1 in the asthma group was higher than in the healthy
control group［（7.497±3.274）ng/mL vs.（4.174±2.122）ng/mL，P < 0.000 1］，and is positively correlated with the level of serum total IgE
（r=0.287，P=0.048）. CHL1 is mainly expressed in bronchial epithelial cells in mouse lung tissue，the expression level of CHL1 in
asthma mice was higher than that in the control group. After stimulation of exogenous TGF⁃β at different times，the mRNA and protein
levels of CHL1 were up⁃regulated. Conclusion：The expression of CHL1 is elevated in asthma，and this process may be related to TGF⁃β
related signal pathways.
［Key words］ asthma；CHL1；bronchial epithelial cell；TGF⁃β
［J Nanjing Med Univ，2022，42（06）：796⁃801］

支气管哮喘是常见的慢性呼吸气道疾病，近年 发病率呈不断上升趋势，流行病学研究表明全球约
有 3.3 亿患者 ［1-2］ 。气道重塑是哮喘重要的病理特
［2］
［基金项目］ 国家自然科学基金面上项目（81870039） 征，且与哮喘不良预后有关 。研究表明，上皮间充
∗ 质转化（epithelial mesenchymal transition，EMT），即
通信作者（Corresponding author），E⁃mail：yaoxin@njmu.edu.cn