Page 58 - 南京医科大学学报自然科学版

南京医科大学学报（自然科学版） 第42卷第8期
·1100 · Journal of Nanjing Medical University（Natural Sciences） 2022年8月

·基础研究·

lncRNA在骨质疏松中作用的初步研究

李北辰 ，徐芳蓉 ，杨崇正 ，刘 衡 ，孙 强 1*
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南京医科大学附属南京医院脊柱外科，江苏 南京 210006；南京医科大学人体解剖学系，江苏 南京 211166
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［摘 要］ 目的：探究原发性骨质疏松（osteoporosis，OP）中差异基因及lncRNA RAD51⁃AS1的作用，为原发性骨质疏松的诊断
及治疗提供新思路。方法：利用 GEO（Gene Expression Omnibus）数据库中的 GSE35956 芯片数据进行分析，使用 DEseq2 对
mRNA 进行差异分析获得差异基因（differentially expressed gene，DEG），使用 ClusterProfiler 对差异分析的基因进行富集分析；
随后对芯片进行长链非编码RNA（long non⁃coding RNA，lncRNA）重注释以发掘差异lncRNA，运用实时定量PCR（qRT⁃PCR）对
正常人及骨质疏松患者的骨髓间充质干细胞（human bone marrow mesenchymal stem cell，hBMSC）进行表达量验证。最后用
MTT、克隆形成和ALP染色观察RAD51⁃AS1对hBMSC增殖及成骨分化的影响。结果：共获得关于mRNA的1 440个DEG，其功
能主要富集于受体配体活性和单核细胞分化等过程。同时也获得关于lncRNA的105个DEG，发现RAD51⁃AS1在骨质疏松患
者中表达显著下调。沉默 RAD51⁃AS1 后发现 hBMSC 的增殖减少和 ALP 活性下降。结论：PI3K⁃Akt 信号转导途径和单核细
胞分化在骨质疏松中发挥重要作用。lncRNA RAD51⁃AS1 可以调节骨髓间充质干细胞增殖及分化，以缓解骨质疏松进程。
［关键词］ 骨质疏松；生物信息学；长链非编码RNA；RAD51⁃AS1
［中图分类号］ R681 ［文献标志码］ A ［文章编号］ 1007⁃4368（2022）08⁃1100⁃07
doi：10.7655/NYDXBNS20220808

Prelimnary study one the role of lncRNA in osteoporosis

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LI Beichen ，XU Fangrong ，YANG Chongzheng ，LIU Heng ，SUN Qiang
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1 Department of Orthopedics，Nanjing Hospital Affiliated to Nanjing Medical University，Nanjing 210006；Department
of Human Anatomy，Nanjing Medical University，Nanjing 211166，China

［Abstract］ Objective：To investigate the role of differential genes and lncRNA RAD51⁃AS1 in primary osteoporosis（OP），which
provides new insights for the diagnosis and treatment of primary osteoporosis. Methods：The GSE35956 microarray data from the GEO
database was used for analysis. Differentially expressed genes（DEG）were obtained by using the DEseq2，and the ClusterProfiler was
used to find the enrichment of DEGs. The microarrays were then reannotated to discover the differential long ⁃ non coding RNA
（lncRNA）in OP. The expression of RAD51⁃AS1 in hBMSC was verified by qRT⁃PCR. Finally，the effects of RAD51⁃AS1 on hBMSC
proliferation and osteogenic differentiation were examined by MTT，colony formation，and ALP staining. Results：A total of 1 440 DEG
was obtained about mRNAs in this study，whose functions were mainly enriched in processes such as receptor⁃ligand activity and
monocyte differentiation. A total of 105 DEG regarding lncRNAs were also acquired. We found that RAD51 ⁃ AS1 expression was
significantly downregulated in osteoporosis. The proliferation and ALP activity of hBMSC was reduced after silencing RAD51⁃AS1.
Conclusions：The PI3K⁃Akt signaling pathway and monocyte differentiation plays important roles in osteoporosis by bioinformatics.
LncRNA RAD51 ⁃ AS1 regulates the proliferation and differentiation of bone marrow mesenchymal stem cells to ameliorate the
progression of osteoporosisby cytological verification.
［Key words］ osteoporosis；bioinformatics；long non⁃coding RNA；RAD51⁃AS1
［J Nanjing Med Univ，2022，42（08）：1100⁃1106］

［基金项目］ 南京市卫生科技发展专项资金项目（ZKX14032，ZKX19025）
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通信作者（Corresponding author），E⁃mail：sunqiang_cn@njmu.edu.cn