Page 17 - 南京医科大学自然版

第44卷第11期 南京医科大学学报（自然科学版）
2024年11月 Journal of Nanjing Medical University（Natural Sciences） ·1483 ·

·基础研究·

mfat⁃1转基因小鼠通过促进神经干细胞增殖修复缺氧缺血性脑

损伤

刘雨桐，张 何，蔡皓然，陈雨沐，杨海元 ，王 盈 *
*
江苏省异种移植重点实验室，南京医科大学医学遗传学系，江苏 南京 211166

［摘 要］ 目的：验证mfat⁃1转基因小鼠是否通过促进成体神经干细胞增殖修复缺氧缺血性脑损伤。方法：在体外实验中，分
离并培养 mfat⁃1 转基因小鼠和同窝阴性鼠的成体神经干细胞，进行氧糖剥夺/复氧（oxygen⁃glucose deprivation/reoxygenation，
OGD/R）造模，检测神经干细胞增殖情况；在体内实验中，对mfat⁃1转基因小鼠及其同窝阴性对照小鼠进行缺氧缺血性脑损伤
（hypoxic⁃ischemic brain damage，HIBD）造模，检测成体神经干细胞的增殖能力。结果：成功构建HIBD小鼠模型和神经干细胞
体外培养体系。mfat⁃1转基因小鼠来源的成体神经干细胞的增殖能力较野生型小鼠来源的成体神经干细胞更强；与野生型小
鼠比较，mfat⁃1转基因小鼠神经干细胞增殖能力显著增强且行为学改善。结论：mfat⁃1转基因小鼠通过促进神经干细胞增殖修
复小鼠HIBD。
［关键词］ mfat⁃1；成体神经干细胞；缺氧缺血性脑损伤；增殖
［中图分类号］ R743.3 ［文献标志码］ A ［文章编号］ 1007⁃4368（2024）11⁃1483⁃08
doi：10.7655/NYDXBNSN240442

Mfat ⁃ 1 transgenic mice participate in the repair of hypoxic ⁃ ischemic brain injury by
promoting the proliferation of neural stem cells

* *
LIU Yutong，ZHANG He，CAI Haoran，CHEN Yumu，YANG Haiyuan ，WANG Ying
Jiangsu Key Laboratory of Xenotransplantation，Department of Medical Genetics，Nanjing Medical University，
Nanjing 211166，China

［Abstract］ Objective：To verify whether mfat⁃ 1 transgenic mice participate in the repair of hypoxic⁃ischemic brain damage by
promoting the proliferation of adult neural stem cells. Methods：In vitro experiments，adult neural stem cells from wild⁃type and mfat⁃1
transgenic mice were isolated and cultured，subjected to oxygen ⁃ glucose deprivation/reoxygenation（OGD/R）modeling，and the
proliferation of neural stem cells was assessed；in vivo experiments，mfat⁃1 transgenic mice and their littermates were subjected to
hypoxic⁃ischemic brain damage（HIBD）modeling，and the proliferation capacity of adult neural stem cells was evaluated. Results：The
mouse HIBD model and the neural stem cell in vitro culture system were established successfully. The proliferation ability of adult
neural stem cells derived from mfat⁃ 1 transgenic mice was higher than that from wild⁃type mice；mfat⁃ 1 transgenic mice showed
significantly enhanced proliferation capacity of neural stem cells compared to wild ⁃ type mice and displayed better behavioral
performance. Conclusion：mfat⁃1 transgenic mice participate in the repair of HIBD by promoting the proliferation of mouse neural stem
cells.
［Key words］ mfat⁃1；adult neural stem cell；hypoxic⁃ischemic brain damage；proliferation
［J Nanjing Med Univ，2024，44（11）：1483⁃1490］

［基金项目］ 国家自然科学基金（31701283，81970164）；江苏省高等学校大学生创新创业训练计划项目（202310312078Y）
∗
通信作者（Corresponding author），E⁃mail：hyyang@njmu.edu.cn；ywang@njmu.edu.cn