Page 23 - 南京医科大学自然版

第45卷第8期 喻琦钦，杨泽昕，张俊麒，等. 同种异体小鼠肾移植慢性抗体介导排斥反应模型的构建与鉴定［J］.
2025年8月 南京医科大学学报（自然科学版），2025，45（8）：1073-1081 ·1079 ·

A B C D
aABMR cABMR
aABMR 800 8
（×400） 3 * * * 600 ** 6 *
4
cABMR
HE Banff scores 2 （μmol/L） 400 （mmol/L） 4

（×400） 1 0 i t v g ptc Cr 200 0 aABMR cABMR BUN 2 0 aABMR cABMR

PAS

E 15 F 50 000 G H aABMR cABMR
（%） 10 * 40 000 *
C3d positive area 5 Mean fluorescence intensity 30 000 aABMR CD19

20 000
10 000
0
F4/80
aABMR cABMR 0 aABMR cABMR
（%） 3 （%） 8 6 *
I J cABMR
positive area 2 1 positive area 4 CD3

CD19 0 F4/80 2 0
Ly6G
aABMR cABMR aABMR cABMR
K L M
（%） 3 （%） 2.5 * 15
2.0
positive area 2 1 Ly6G positive area 1.5 Foxp3 relative mRNA expession 10 5 ***
1.0
CD3 0 0.5 0 0
aABMR cABMR aABMR cABMR aABMR cABMR

N 8 O 200 * P 50 ** Q 80 ** R 80
relative mRNA expession 4 2 IL⁃1β relative mRNA expession 100 relative mRNA expession 20 relative mRNA expession 40 CCL2 relative mRNA expession 40

30
IL⁃10 6 ** 150 IL⁃6 40 TNF⁃α 60 60 **
20
50
20
10
0
aABMR cABMR 0 aABMR cABMR 0 aABMR cABMR 0 aABMR cABMR 0 aABMR cABMR
A：HE and PAS staining results of transplanted kidneys of aABMR and cABMR（×400）. B：Banff scores of transplanted kidneys of aABMR and
cABMR. C：Peripheral blood serum Cr expression levels of aABMR and cABMR. D：Peripheral blood serum Bun expression levels of aABMR and
cABMR. E：Results of semi⁃quantitative analysis of C3d complement⁃positive regions in transplanted kidneys of aABMR and cABMR. F：Peripheral
blood IgG class DSA expression level of aABMR and cABMR. G：Immunohistofluorescence staining results of C3d complement in transplanted kidneys
of aABMR and cABMR（×400）. H：Immunohistochemical staining results of CD19，F4/80，CD3 and Ly6G in transplanted kidneys of aABMR and
cABMR（×400）. I：Results of semi⁃quantitative analysis of CD19⁃positive regions in transplanted kidneys of aABMR and cABMR. J：Results of semi⁃
quantitative analysis of F4/80⁃positive regions in transplanted kidneys of aABMR and cABMR. K：Results of semi⁃quantitative analysis of CD3⁃positive
regions in transplanted kidneys of aABMR and cABMR. L：Results of semi⁃quantitative analysis of Ly6G⁃positive regions in transplanted kidneys of
aABMR and cABMR. M-R：qRT⁃PCR detection of Foxp3，IL⁃10，IL⁃1β，IL⁃6，TNF⁃α and CCL2 mRNA expression levels in transplanted kidneys of
**
*
aABMR and cABMR. Compared to aABMR，P < 0.05，P < 0.01（n=10）.
图5 aABMR与cABMR模型比较
Figure 5 Comparison of aABMR and cABMR models