Page 14 - 南京医科大学学报自然科学版

南京医科大学学报（自然科学版） 第43卷第3期
·304 · Journal of Nanjing Medical University（Natural Sciences） 2023年3月

·基础研究·

LINC01518对IL⁃17诱导非小细胞肺癌细胞增殖的影响

阮玉婷 ，李 雅 ，葛 文 ，吴宁霞 ，应 帅 ，王伟民 ，李 玉 ，张 婧 ，邱 文 ，王迎伟 ，赵晨卉 2*
1
1
1
1
1
1
1
1
1
1*
南京医科大学免疫学系，江苏 南京 211166；南京医科大学第一附属医院肿瘤科，江苏 南京 210029
1 2
［摘 要］ 目的：探讨长链非编码RNA01518（long intergenic non⁃protein coding RNA 01518，LINC01518）基因过表达或沉默对
白介素（interleukin，IL）⁃17 诱导非小细胞肺癌（non⁃small cell lung cancer，NSCLC）细胞增殖的影响。方法：Western blot 检测
NSCLC 细胞系（PC9、H1299和H1975）IL⁃17受体A（IL⁃17 receptor A，IL⁃17RA）的表达后，用IL⁃17刺激H1299和PC9细胞不同
时间，利用CCK⁃8实验测定细胞的增殖水平。LncRNA芯片筛查IL⁃17刺激H1299和PC9细胞3 h后LncRNA上调的结果，从中
选取某些上调的LncRNA进行RT⁃PCR和Real⁃time PCR验证。此外，将构建的pcDNA3.1/LINC01518或shLINC01518质粒转染
H1299细胞，用CCK⁃8、EdU和克隆形成实验检查细胞的增殖情况。结果：3种NSCLC细胞系均有IL⁃17RA的表达。实验发现，
IL⁃17刺激H1299和PC9细胞后能提高其增殖水平，同时受刺激的细胞内LINC01518的上调最为显著，且过表达LINC01518可
提高H1299细胞的增殖水平，而沉默LINC01518基因则能抑制IL⁃17诱导的细胞增殖。结论：LINC01518能促进IL⁃17诱导的
H1299细胞增殖。
［关键词］ NSCLC；IL⁃17；LINC01518；细胞增殖
［中图分类号］ R734.2 ［文献标志码］ A ［文章编号］ 1007⁃4368（2023）03⁃304⁃07
doi：10.7655/NYDXBNS20230302

Effects of LINC01518 on the proliferation of non⁃small cell lung cancer cells stimulated

with IL⁃17
RUAN Yuting ，LI Ya ，GE Wen ，WU Ningxia ，YING Shuai ，WANG Weimin ，LI Yu ，ZHANG Jing ，QIU Wen ，
1
1
1
1
1
1
1
1
1
1*
WANG Yingwei ，ZHAO Chenhui 2*
1 2
Department of Immunology，Nanjing Medical University，Nanjing 211166；Department of Oncology，the First
Affiliated Hospital of Nanjing Medical University，Nanjing 210029，China
［Abstract］ Objective：This study aims to explore the effect of overexpressing or silencing long intergenic non⁃protein coding
RNA 01518（LINC01518）on the cell proliferation induced by IL⁃17 in non⁃small cell lung cancer（NSCLC）. Methods：The IL⁃17
receptor A（IL⁃17RA）expression in NSCLC cell lines（PC9，H1299 and H1975）was first examined using Western blot. Then H1299
and PC9 cells were exposed to IL⁃17 for different time，and the cell proliferation was detected by CCK⁃8 assay. Based on the results of
up⁃regulated lncRNAs detected by lncRNA microarray in IL⁃17⁃stimulated H1299 and PC9 cells，several lncRNAs were selected and
verified by RT ⁃ PCR and Real ⁃ time PCR. Additionally，the constructed plasmids of pcDNA3.1/LINC01518 or shLINC01518 were
transfected into H1299 cells，and H1299 cell proliferation were examined by CCK⁃8，EdU and colony formation assays. Results：
IL⁃17RA could express in 3 kinds of NSCLC cell lines. The cell proliferation and LINC01518 were obviously up⁃regulated in H1299
and PC9 cells treated by IL⁃17. Besides，LINC01518 overexpression could markedly promote the cell proliferation，while LINC01518
gene knockdown followed by IL⁃17 treatment did not enhance cell proliferation. Conclusion：LINC01518 can promote H1299 cell
proliferation induced by IL⁃17 stimulation.
［Key words］ NSCLC；IL⁃17；LINC01518；cell proliferation
［J Nanjing Med Univ，2023，43（03）：304⁃310］

［基金项目］ 国家自然科学基金（81902878，81971468）
∗
通信作者（Corresponding author），E⁃mail：wangyw1508@njmu.edu.cn；candy9023@163.com